A7050 Cortactin (Phospho-Tyr466) Antibody_详细资料

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A7050   Cortactin （Phospho-Tyr466） Antibody

Reactivity Applications MW. Source

M, R WB IHC ELISA 61 kDa Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name Cortactin （Phospho-Tyr466） Antibody

Synonyms Src substrate cortactin; Amplaxin; EMS1; CTTN; EMS1

Immunogen The antiserum was produced against synthesized peptide derived from mouse Cortactin around the phosphorylation site of Tyr466.

Immunogen Region C-terminal

Clonality Polyclonal

Modification sites M:Y466 R:Y429

Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000

Specificity Cortactin （Phospho-Tyr466） Antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine466.

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.

Physical Form Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

Concentration 1 mg/ml

Storage/Stability Store at -20℃/1 year

NCBI Gene Symbol CTTN

Database Links

Gene ID: 13043 Swiss-Prot #: Q60598

OMIM #: Unigene #: Mm.205601

Application images
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Cortactin （Phospho-Tyr466） Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from 293 cells, using Cortactin （Phospho-Tyr466） Antibody. The lane on the right is treated with the synthesized peptide.

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Cortactin （Phospho-Tyr466） Antibody

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Product Name	Cortactin （Phospho-Tyr466） Antibody
Synonyms	Src substrate cortactin; Amplaxin; EMS1; CTTN; EMS1
Immunogen	The antiserum was produced against synthesized peptide derived from mouse Cortactin around the phosphorylation site of Tyr466.
Immunogen Region	C-terminal
Clonality	Polyclonal
Modification sites	M:Y466 R:Y429
Application dilutions	WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:20000
Specificity	Cortactin （Phospho-Tyr466） Antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine466.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form	Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year