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A7049 Cortactin (Phospho-Tyr421) Antibody
Reactivity | Applications | MW. | Source |
H, M, R | WB ELISA | 61 kDa | Rabbit |
Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.
Product Name | Cortactin (Phospho-Tyr421) Antibody |
Synonyms | Src substrate cortactin; Amplaxin; EMS1; CTTN; EMS1 |
Immunogen | The antiserum was produced against synthesized peptide derived from human Cortactin around the phosphorylation site of Tyr421. |
Immunogen Region | Internal |
Clonality | Polyclonal |
Modification sites | H:Y421 M:Y421 R:Y384 |
Application dilutions | WB: 1:500~1:1000 ELISA: 1:20000 |
Specificity | Cortactin (Phospho-Tyr421) Antibody detects endogenous levels of Cortactin only when phosphorylated at tyrosine421. |
Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. |
Physical Form | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration | 1 mg/ml |
Storage/Stability | Store at -20℃/1 year |
NCBI Gene Symbol | CTTN |
Database Links | Gene ID: | 2017 | Swiss-Prot #: | Q14247 | OMIM #: | 164765 | Unigene #: | Hs.596164 | |
Application images | Western blot analysis of extracts from HeLa cells treated with H2O2, using Cortactin (Phospho-Tyr421) Antibody. The lane on the right is treated with the synthesized peptide. |
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