A7171 NF-kappaB p65 (Phospho-Ser536) Antibody_详细资料

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A7171    NF-kappaB p65 （Phospho-Ser536） Antibody

Reactivity Applications MW. Source

H, M, R WB IHC IP ELISA 60 kDa Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name NF-kappaB p65 （Phospho-Ser536） Antibody

Synonyms NFKB3; nuclear factor NF-kappa-B p65 subunit; p65; RELA; TF65; transcription factor p65

Immunogen The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser536.

Immunogen Region C-terminal

Clonality Polyclonal

Modification sites H:S536 M:S534 R:S535

Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000

Specificity NF-kappaB p65 （Phospho-Ser536） Antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at serine536.

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.

Physical Form Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

Concentration 1 mg/ml

Storage/Stability Store at -20℃/1 year

NCBI Gene Symbol RELA

Database Links

Gene ID: 5970 Swiss-Prot #: Q04206

OMIM #: 164014 Unigene #: Hs.502875

Application images
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p65 （Phospho-Ser536） Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from K562 cells ａnd COLO cells, using NF-kappaB p65 （Phospho-Ser536） Antibody. The lane on the right is treated with the synthesized peptide.

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Reactivity	Applications	MW.	Source
H, M, R	WB IHC IP ELISA	60 kDa	Rabbit

Product Name	NF-kappaB p65 （Phospho-Ser536） Antibody
Synonyms	NFKB3; nuclear factor NF-kappa-B p65 subunit; p65; RELA; TF65; transcription factor p65
Immunogen	The antiserum was produced against synthesized peptide derived from human NF-kappaB p65 around the phosphorylation site of Ser536.
Immunogen Region	C-terminal
Clonality	Polyclonal
Modification sites	H:S536 M:S534 R:S535
Application dilutions	WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:10000
Specificity	NF-kappaB p65 （Phospho-Ser536） Antibody detects endogenous levels of NF-kappaB p65 only when phosphorylated at serine536.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form	Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year