IkappaB-epsilon (Phospho-Ser22) Antibody_详细资料

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Reactivity Applications MW. Source

H, M, R WB IHC IF ELISA 38 kDa Rabbit

Note: The product is for research use only, not for use in diagnostic or therapeutic procedures.

Product Name IkappaB-epsilon （Phospho-Ser22） Antibody

Synonyms I-kappa-B-epsilon; IKBE; IkappaBepsilon; NF-kappa-BIE; NF-kappaB inhibitor epsilon; NFKBIE

Immunogen The antiserum was produced against synthesized peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22.

Immunogen Region Internal

Clonality Polyclonal

Modification sites H:S22 M:S22 R:S22

Application dilutions WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000

Specificity IkappaB-epsilon （Phospho-Ser22） Antibody detects endogenous levels of IkappaB-epsilon only when phosphorylated at serine22.

Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.

Physical Form Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.

Concentration 1 mg/ml

Storage/Stability Store at -20℃/1 year

NCBI Gene Symbol NFKBIE

Database Links

Gene ID: 4794 Swiss-Prot #: O00221

OMIM #: 604548 Unigene #: Hs.458276

Application images
Immunofluorescence analysis of HeLa cells, using IkappaB-epsilon （Phospho-Ser22） Antibody. The picture on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IkappaB-epsilon （Phospho-Ser22） Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from Jurkat cells treated with TNF-a 20ng/ml 30＇, using IkappaB-epsilon （Phospho-Ser22） Antibody. The lane on the right is treated with the synthesized peptide.

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Reactivity	Applications	MW.	Source
H, M, R	WB IHC IF ELISA	38 kDa	Rabbit

Product Name	IkappaB-epsilon （Phospho-Ser22） Antibody
Synonyms	I-kappa-B-epsilon; IKBE; IkappaBepsilon; NF-kappa-BIE; NF-kappaB inhibitor epsilon; NFKBIE
Immunogen	The antiserum was produced against synthesized peptide derived from human IkappaB-epsilon around the phosphorylation site of Ser22.
Immunogen Region	Internal
Clonality	Polyclonal
Modification sites	H:S22 M:S22 R:S22
Application dilutions	WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
Specificity	IkappaB-epsilon （Phospho-Ser22） Antibody detects endogenous levels of IkappaB-epsilon only when phosphorylated at serine22.
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Physical Form	Rabbit IgG in phosphate buffered saline （without Mg2+ ａnd Ca2+）, pH 7.4, 150mM NaCl, 0.02% sodium azide ａnd 50% glycerol.
Concentration	1 mg/ml
Storage/Stability	Store at -20℃/1 year