货号:6570
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ScienCell人结膜成纤维细胞 HConF英文说明书:
DescriptionThe fibroblast is the cellular element responsible for fibrosis. Conjunctival fibroblasts, as other fibroblasts, act not only as structural cells in the production of the matrix components, but are also actively involved as effectors in allergic conjunctivitis [1]. Conjunctival fibroblasts cultured with histamine induce the production of proinflammatory cytokines, such as IL-1, IL-6 and IL-8 [2]. Conjunctival fibroblast culture can serve as a useful in vitro model for rapid screening of drugs for their effect on fibroblast morphology, motility, and proliferation, and provide a basis for the development of new treatments for a variety of ocular proliferative disorders.
HConF from ScienCell Research Laboratories are isolated from human conjunctiva. HConF are cryopreserved at primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HConF are characterized by their spindle morphology and immunofluorescent method with antibody to fibronectin. HConF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HConF are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HConF
in vitro.
Product Use
HConF are for research use only. It is not approved for human or animal use, or for application in
in vitrodiagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Shipping
Dry ice.
Publications for Human Conjunctival Fibroblasts1. Swaney, J. S., K. M. Moreno, et al. (2008). "Sphingosine-1-phosphate (S1P) is a novel fibrotic mediator in the eye." Exp Eye Res 87(4): 367-375.
2. Zhang, J. Z., M. E. Cavet, et al. (2009). "BOL-303242-X, a novel selective glucocorticoid receptor agonist, with full anti-inflammatory properties in human ocular cells." Mol Vis 15: 2606-2616.
视觉细胞 相关细胞:
6510 人角膜上皮细胞 (HCEpiF)6520 人角膜细胞 (HK)
6540 人视网膜色素上皮细胞 (HRPEpiC)
6550 人晶状体上皮细胞 (HLEpiC)6560 人虹膜色素上皮细胞 (HIPEpiC)6570 人结膜成纤维细胞(HConF)
6580 人无色素睫状上皮细胞 (HNPCEpiC)6590 人小梁网细胞 (HTMC)6620 人眼球脉络膜成纤维细胞 (HOCF)6630 人结膜上皮细胞 (HConEpiC)