质量标准: | 项目 生化试剂级 BC 纯度assay ≥99%(T) 水分Moistrue ≤0.5% 重金属Heavy Metals <5PPM pH(1% in H2O) 4.7-6.0 三(羟甲基)氨基甲烷盐酸盐(Tris HCl) ,1185-53-1,Tris(hydroxymethyl)aminomethane hydrochloride 项目 超纯级 纯度assay ≥99.0%(T) 水分Moistrue ≤0.5% 铁Iron(Fe) <5PPM 氯Chloride ≤0.005% 硫酸根Sulfate ≤0.005% 重金属Heavy Metals (as Pb) <5PPM 灼烧残渣Residue On Ignition ≤0.1% A260(1M,water): ≤0.025 A280(1M,water): ≤0.020 品级Grade 分子生物学级 (grade for molecular biology) 含量assay ≥99.0% (AT) DNA酶DNases, none detected RNA酶RNases, none detected 不溶物insoluble matter, passes filter test 磷酸酶phosphatases, none detected 蛋白酶proteases, none detected 细胞培养测试cell culture test Pass 灼烧残渣ign. residue ≤0.2% (as SO4) 干燥损失loss on drying ≤0.2% , 110 °C pH 2.5-4.0 (25 °C, 4 M in H2O) useful pH range 7.0 - 9.0 pKa (25 °C) 8.1 水溶性solubility H2O: soluble4 M at 20 °C, clear, colorless 硫酸根sulfate (SO42-): ≤50 ppm Al: ≤5 ppm As: ≤0.1 ppm Ba: ≤5 ppm Bi: ≤5 ppm Ca: ≤10 ppm Cd: ≤5 ppm Co: ≤5 ppm Cr: ≤5 ppm Cu: ≤5 ppm Fe: ≤5 ppm K: ≤50 ppm Li: ≤5 ppm Mg: ≤5 ppm Mn: ≤5 ppm Mo: ≤5 ppm Na: ≤50 ppm Ni: ≤5 ppm Pb: ≤5 ppm Sr: ≤5 ppm Zn: ≤5 ppm λ 4 M in H2O UV absorption 290nm A ≤0.05 AT (40% W/W) 260nm A ≤0.06 AT (1M IN WATER) 280nm A ≤0.06 AT (1M IN WATER |
贮 存: | 三(羟甲基)氨基甲烷盐酸盐(Tris HCl) ,1185-53-1,Tris(hydroxymethyl)aminomethane hydrochloride 室温保存 用途 所有缓冲液的pH值依赖于温度和浓度。对于Tris 缓冲液,温度每增加1°C pH增加大约 0.03 单位, 每10倍的稀释pH减少0.03-0.05 单位。 |