人食道平滑肌细胞

产品：人食道平滑肌细胞
货号：2710
上海复蒙基因生物科技有限公司是美国Sciencell公司ZG代理：
美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在拥有众多客户。在国内销售8年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和的产品品质，深受广大科研工作者的信赖。ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。

产品：人食道平滑肌细胞
货号：2710

平滑肌的主要功能是负责空腔脏器的收缩，如血管、胃肠道、膀胱以及子宫等器官。它的结构与骨骼肌有很大的不同。人食管壁包括三层，外纵形和内环形，中间肌层是黏膜层。人食管的蠕动是由平滑肌收缩严格控制。平滑肌的收缩可能是通过两条不同的途径激活磷脂酶（增加细胞内Ca²⁺浓度和激活G蛋白）并激活PKC-epsilon依赖的信号通路。在体外培养研究表明人食管平滑肌细胞可以表达Na^（+）通道。

    人食道平滑肌细胞 （HESMC）提取于人食道组织，原代冻存。每管含有细胞数>5×10⁵ cells/ml，此细胞通过对alpha-smooth muscle actin和 desmin免疫荧光染色验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。

推荐培养基:（SMCM, Cat. No. 1101）

产地：San Diego,US储存：

品Pai：ScienCell

运输：干冰

用途：科研

Smooth muscle is responsible for the contractility of hollow organs, such as blood vessels, the gastrointestinal tract, the bladder, ａnd the uterus. Its structure differs greatly from that of skeletal muscle. The human esophagus contains three layers of muscle in its walls, the outer longitudinal ａnd inner circular layers of the main muscular coat ａnd the muscular layer of the mucosa. Visceral smooth muscle cells makes up all three layers along the entire organ except for the most rostral few centimeters. Smooth muscle contraction is critical to peristalsis in the human esophagus ａnd the contraction may be mediated by activation of phospholipase through two distinct mechanisms （increased intracellular Ca2+ ａnd G protein activation） ａnd activating PKCepsilon-dependent mechanisms [1]. In vitro study also shows that cultured human esophageal smooth muscle cells express voltage-dependent Na（+） channels [2].

HESMC from ScienCell Research Laboratories are isolated from human esophagus. HESMC are cryopreserved at secondary culture ａnd delivered frozen. Each vial contains >5 x 10⁵ cells in 1 ml volume. HESMC are characterized by immunofluorescent method with antibodies to alpha-smooth muscle actin ａnd desmin. HESMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast ａnd fungi. HESMC are guaranteed to further expand for 15 population doublings at the condition provided by ScienCell Research Laboratories.

Reference
[1]. Cao, W., Chen, Q., Sohn, U. D., Kim, N., Kirber, M. T., Harnett, K. M., Behar, J., Biancani, P. （2001） Ca2+-induced contraction of cat esophageal circular smooth muscle cells. Am J Physiol Cell Physiol. 280（4）:C980-92.
[2]. Deshpande, M. A., Wang, J., Preiksaitis, H. G., Laurier, L. G., Sims, S. M. （2002） Characterization of a voltage-dependent Na（+） current in human esophageal smooth muscle. Am J Physiol Cell Physiol. 283（4）:C1045-55.