PRO-623 NusA E.Coli/重组大肠杆菌转录终止/抗终止L因子_详细资料

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NusA E.Coli/重组大肠杆菌转录终止/抗终止L因子
产品编号：PRO-623
产品规格：20μg/100μg/1mg

Synonyms Transcription elongation protein nusA, N utilization substance protein A, L factor, nusA, ECK3158, JW3158, b3169, Transcription Termination/Antitermination L Factor.

Introduction NusA is an important player in both prevention ａnd enhancement of transcriptional termination. NusA is important both in Rho-dependent ａnd intrinsic termination, as well as in lambda ａnd other phage antitermination systems. The NusA gene was first identified by isolation of the nusAl mutation, which limits bacteriophage-l growth by preventing the antitermination activity of the l N protein. NusA plays a role in transcriptional antitermination in the cell. It has been shown to specifically aid in read-through of the RNA polymerase genes rpoB ａnd rpoC, as well as in successful synthesis of the ribosomal RNA genes. Additionally to its anti-termination role, NusA is needed for both Rho-dependent ａnd intrinsic transcriptional termination. NusA is obligatory for Rho-dependent termination in lambda phage ａnd in the cell. NusA plays a role in intrinsic termination ａnd the inhibition of RNA elongation. However NusA interacts with all three subunits of RNA polymerase, its termination activity primarily depends on its interaction with the carboxy-terminus of RpoA. NusA induces conformational change in RNA polymerase & prevents RNA interaction with RpoA. This binding sequentially activates NusA, allowing it to bind RNA ａnd promote formation of hairpins at intrinsic termination sites. NusA binds Rho, ａnd participates with sigma70 for binding to the core RNA polymerase complex. NusA does not compete with NusG for binding to either Rho or the polymerase, despite modulating the same process as NusG in both cases.

Description NusA Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 495 amino acids ａnd having a molecular mass of 54 kDa.

Source Escherichia Coli.

Physical Appearance Sterile Filtered colorless solution.

Formulation NusA protein solution contains 1x PBS pH-7.4.

Stability NusA although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.

Amino Acid Sequence MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFRRWLVVDEVTQ PTKEITLEAA RYEDESLNLG DYVEDQIESV TFDRITTQTA KQVIVQKVREAERAMVVDQF REHEGEIITG VVKKVNRDNI SLDLGNNAEA VILREDMLPR ENFRPGDRVR GVLYSVRPEA RGAQLFVTRS KPEMLIELFR IEVPEIGEEV IEIKAAARDP
GSRAKIAVKT NDKRIDPVGA CVGMRGARVQ AVSTELGGER IDIVLWDDNP AQFVINAMAP ADVASIVVDE DKHTMDIAVE AGNLAQAIGR NGQNVRLASQ LSGWELNVMT DDLQAKHQA EAHAAIDTFT KYLDIDEDFA TVLVEEGFST LEELAYVPMK
ELLEIEGLDE PTVEALRERA KNALATIAQA QEESLGDNKP ADDLLNLEGV DRDLAFKLAA RGVCTLEDLA EQGIDDLADI EGLTDEKAGA LIMAARNICW FGDEA.

Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.

Usage ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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Synonyms	Transcription elongation protein nusA, N utilization substance protein A, L factor, nusA, ECK3158, JW3158, b3169, Transcription Termination/Antitermination L Factor.
Introduction	NusA is an important player in both prevention ａnd enhancement of transcriptional termination. NusA is important both in Rho-dependent ａnd intrinsic termination, as well as in lambda ａnd other phage antitermination systems. The NusA gene was first identified by isolation of the nusAl mutation, which limits bacteriophage-l growth by preventing the antitermination activity of the l N protein. NusA plays a role in transcriptional antitermination in the cell. It has been shown to specifically aid in read-through of the RNA polymerase genes rpoB ａnd rpoC, as well as in successful synthesis of the ribosomal RNA genes. Additionally to its anti-termination role, NusA is needed for both Rho-dependent ａnd intrinsic transcriptional termination. NusA is obligatory for Rho-dependent termination in lambda phage ａnd in the cell. NusA plays a role in intrinsic termination ａnd the inhibition of RNA elongation. However NusA interacts with all three subunits of RNA polymerase, its termination activity primarily depends on its interaction with the carboxy-terminus of RpoA. NusA induces conformational change in RNA polymerase & prevents RNA interaction with RpoA. This binding sequentially activates NusA, allowing it to bind RNA ａnd promote formation of hairpins at intrinsic termination sites. NusA binds Rho, ａnd participates with sigma70 for binding to the core RNA polymerase complex. NusA does not compete with NusG for binding to either Rho or the polymerase, despite modulating the same process as NusG in both cases.
Description	NusA Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 495 amino acids ａnd having a molecular mass of 54 kDa.
Source	Escherichia Coli.
Physical Appearance	Sterile Filtered colorless solution.
Formulation	NusA protein solution contains 1x PBS pH-7.4.
Stability	NusA although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）. Please prevent freeze-thaw cycles.
Amino Acid Sequence	MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFRRWLVVDEVTQ PTKEITLEAA RYEDESLNLG DYVEDQIESV TFDRITTQTA KQVIVQKVREAERAMVVDQF REHEGEIITG VVKKVNRDNI SLDLGNNAEA VILREDMLPR ENFRPGDRVR GVLYSVRPEA RGAQLFVTRS KPEMLIELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT NDKRIDPVGA CVGMRGARVQ AVSTELGGER IDIVLWDDNP AQFVINAMAP ADVASIVVDE DKHTMDIAVE AGNLAQAIGR NGQNVRLASQ LSGWELNVMT DDLQAKHQA EAHAAIDTFT KYLDIDEDFA TVLVEEGFST LEELAYVPMK ELLEIEGLDE PTVEALRERA KNALATIAQA QEESLGDNKP ADDLLNLEGV DRDLAFKLAA RGVCTLEDLA EQGIDDLADI EGLTDEKAGA LIMAARNICW FGDEA.
Purity	Greater than 95.0% as determined by（a） Analysis by RP-HPLC. （b） Analysis by SDS-PAGE.
Usage	ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.