Neutrophil Cytosolic Factor 1 Human Recombinant(NCF1 Human)/重组人中性粒细胞cytosolic因子1

Synonyms

NCF1A, NOXO2, p47phox, SH3PXD1A .

Introduction

NCF1 is a cytosolic subunit protein of neutrophil NADPH oxidase which a
Multi-component enzyme that is activates production of superoxide anion. NCF1, along with NCF2 ａnd a membrane bound cytochrome b558, is necessary for activation of the latent NADPH oxidase necessary for superoxide production. Mutations in this NCF1 have been related with chronic granulomatous disease.

Description

NCF1 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 398 amino acids （1-390 a.a.） ａnd having a molecular mass of 45.7 kDa. The NCF1 is fused to 8 amino acid His-Tag at C-terminus ａnd purified by proprietary chromatographic techniques.

Source

Escherichia Coli.

Physical Appearence

Sterile filtered colorless solution.

Formulation

0.5mg/ml solution containing 20mM Tris pH-8, 1mM DTT, 0.1M NaCl & 20% glycerol.

Stability

NCF1 Human although stable at 4C for 1 week, should be stored desiccated
below -18C. Please prevent freeze thaw cycles.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Amino Acid Sequence

MGDTFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF HKTLKEMFPI EAGAINPENR IIPHLPAPKW FDGQRAAENR QGTLTEYCST LMSLPTKISR CPHLLDFFKV RPDDLKLPTD NQTKKPETYL MPKDGKSTAT DITGPIILQT YRAIANYEKT SGSEMALSTG DVVEVVEKSE SGWWFCQMKA KRGWIPASFL EPLDSPDETE DPEPNYAGEP YVAIKAYTAV EGDEVSLLEG EAVEVIHKLL DGWWVIRKDDVTGYFPSMYL QKSGQDVSQA QRQIKRGAPP RRSSIRNAHS IHQRSRKRLS QDAYRRNSVR FLQQRRRQAR PGPQSPGSPL EEERQTQRSK PQPAVPPRPS ADLILNRCSE STKRKLASAV VEHHHHHH.

Usage

ProSpec＇s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.