Sigma F7250 100mg-FITC;Fluorescein Isothiocyanate;异硫氰酸荧光素

异硫氰酸荧光素 (fluorescein isothiocyanate, FITC) FITC纯品为黄色或橙黄色结晶粉末，易溶于水和酒精溶剂。有两种异构体，其中异构体Ⅰ型在效率、稳定性与蛋白质结合力等方面都更优良。FITC分子量为389.4，Z大吸收光波长为490～495nm，Z大发射光波长为520～530nm，呈现明亮的黄绿色荧光。FITC在冷暗干燥处可保存多年，是目前应用广泛的荧光素。其主要优点是人眼对黄绿色较为敏感，通常切片标本中的绿色荧光少于红色。DescriptionApplication Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm.PropertiesSigma  F7250  Fluorescein isothiocyanate isomer Isuitable for protein labeling, ≥90% (HPLC), powderSynonym: FITC, Fluorescein 5-isothiocyanateMolecular Weight: 389.38Beilstein Registry Number: 1407295CAS Number: 3326-32-7Linear Formula: C21H11NO5SEC Number: 222-042-0MDL number: MFCD00005063PubChem Substance ID: 24894933 assay: ≥90% (HPLC)form powderfluorescence λex 492 nm; λem 518 nm (green)mp >360 °C(lit.)suitability suitable for protein labelingstorage temp. 2-8°CSafetyPersonal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, GlovesHazard Codes XnRisk Statements 42/43Safety Statements 22-36/37-45WGK Germany 3