ApplicationProtein-structure and sequence analysis. Suited for the digestion of proteins in polyacrylamide gels. QualityFree of impurities that may interfere with the separation of peptides in RP HPLC. Purity is checked by HPLC and SDS-PAGE using silver staining. Product DescriptionEC 3.4.21.50Inhibitors: DFP, TLCK, aprotinin, and leupeptinMolecular weight: Mr = 33 kD (reduced), Mr = 30 kD (non-reduced)Optimum pH: 8.5 - 8.8Specificity: Serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys.Stability: stable in 4 M urea