99473 Irvine CHANG Amnio 羊水细胞培养基,完全型

美国Irvine公司专业开发细胞培养、人类辅助生殖技术、细胞遗传学和细胞ZL产品。其羊水培养基与同类产品相比，能以更快速度扩增细胞，减少收获时间。

CHANG Amnio®羊水培养基

产品英文名称：CHANG Amnio®

产品中文名称：CHANG Amnio®羊水培养基

应用：羊水细胞原代培养，绒毛膜样本和组织（POC）培养

CHANG Amnio羊水培养基是成分完全的即用型培养基。含抗生素优化用于人羊水细胞、绒毛膜样本和染色体核型分析组织培养等产前检测。CHANG Amnio羊水培养基混合的基础培养基和血清经过改良，比现有其他商用羊水培养基更高产。

订购信息

Irvine CHANG Amnio 羊水细胞培养基,完全型现货数量有限 详询华雅生物集团旗下公司：红荣微再（上海）生物工程技术有限公司  ：1500 1904 520。红荣微再-客服:  经销商专员

CHANG Amnio羊水培养基产品参数

形态：液体

颜色：橙红色

pH值：中性

成分：含抗生素 和L-Glutamine

适用：

the primary culture of amniotic fluid cells 羊水细胞原代培养

growing passaged amniotic fluid cells 羊水细胞传代培养

solid amnion tissue from chorionic villi sampling绒毛膜样本

CHANG Amnio羊水培养基特点

Higher mitotic index 较高的有丝分裂指数

Superior chromosomal morphology 的染色体形态

Low days to harvest 收获时间短

CHANG Amnio羊水培养基培养性能

AFC细胞培养6天后，细胞密度超同类产品。

Low passage cells from pooled amniotic fluid specimens were cultured in 3  different media: Chang Amnio™, Amniomax™II and Amniomax™ C-100. The cells were seeded at 1X104 cells/well in triplicate in a 6-well-dish and grown without medium renewal for 6 days.

CHANG Amnio羊水培养基储存和稳定性

CHANG Amnio®羊水培养基储存在-10°C，避光。。一次性用不完可以再冷冻或者存于2- 8°C，羊水培养基Z多可冻融2次，或者在2- 8°C储存Z多30天。

CHANG Amnio培养基源于1982年Chang等人对产前检测细胞和组织培养的贡献，经过多年发展不断优化。

CHANG Amnio® is a complete, ready-to-use medium with an antibiotic developed for the culture of human amniotic fluid cells, chorionic villi sampling, and tissues for use in karyotyping and other antenatal testing. With an improved blend of basal media and serum, CHANG Amnio has been shown to yield higher growth than other commercially available media.

A variety of invasive and non-invasive techniques are used for successful prenatal diagnosis to monitor fetal gestation and detect fetal abnormalities. Chromosomes from Amniotic Fluid Cell (AFC), chorionic villi sampling (CVS) and tissues (POC) cultured in vitro have been used for more than 40 years to diagnose prenatal genetic abnormalities. A significant advance was reported by Chang et al. (PNAS, 1982) which subsequently led to the development of CHANG MEDIUM®, specifically designed for the culture of prenatal cells and tissues. Both the methods and the nutrient media used in the culture of these cells have evolved considerably over the years. To achieve optimum efficiency for diagnosis, a culture medium needs to promote rapid cell growth for a large number of colonies, fewer days to harvest, deliver a high mitotic index with sufficient metaphase cells and good chromosome morphology.

Chang Amnio™ is a new, high performing complete medium designed and optimized for providing analyzable metaphases for amniotic fluid, chorionic villi sampling and tissue (POC) cultures. It provides a reliable and consistent number of mitotic cells suitable for karyotyping for more accurate cytogenetic analysis. Chang Amnio™ is an excellent medium to choose when culturing prenatal cells.

CHANGAmnio®是一种完全的即用型培养基，含有抗生素，用于培养人类羊水细胞，绒毛膜绒毛取样和用于核型分析和其他产前检测的组织。凭借基础培养基和血清的改良混合物，CHANG Amnio已被证明比其他商用培养基具有更高的生长速度。

多种有创和无创技术成功用于产前诊断，以监测胎儿妊娠并检测胎儿异常。来自羊水细胞（AFC），体外培养的绒毛膜绒毛取样（CVS）和组织（POC）的染色体已用于诊断出生前遗传异常超过40年。 Chang等人报道了一项重大进展（PNAS，1982），在此基础上开发了CHANG MEDIUM®，专门设计用于产前细胞和组织的培养。多年来，这种细胞培养方法和培养基都有了很大的发展。为了达到Z佳的诊断效率，培养基需具备促进细胞快速生长形成集落的能力，更少的收获时间，提供具有充足的中期细胞和良好的染色体形态的高有丝分裂指数。

Chang Amnio™是一种新型，高性能的完全培养基，专为羊水，绒毛绒毛取样和组织（POC）培养设计优化提供可分析的中期细胞。它提供了可靠和一致数量的有丝分裂细胞，适合用于更准确的细胞遗传学核型分析。 Chang Amnio™是培养产前细胞的理想培养基。

CHANG Amnio羊水培养基使用注意

使用产品前操作人员应进行培训，熟练掌握后再进行试验。

试验需要在无菌的条件下进行。

CHANG Amnio羊水培养基使用前准备

在37°C水浴锅边溶解边摇晃以便快速解冻；

CHANG Amnio®含抗生素，可根据需要自行添加抗生素。

分装

• 按照操作说明解冻；
• 在无菌的条件下分装到适用的体积并冷冻；
• 使用前将分装好的小包装37°C水浴即可。

CHANG Amnio羊水培养基使用说明

Use of CHANG Amnio® for Primary Cultures: in situ Methodologies

原代培养：原位法

1. Centrifuge amniotic fluid at low speed to concentrate the cells.

2. Resuspend the cell pellet in a small volume of the patient’s own amniotic fluid.For example, aspirate the supernatant of 10 mL of spun amniotic fluid to 0.5mL above the cell pellet and resuspend. Add sufficient CHANG Amnio® to the concentrated cell suspension to allow for final plating volume of 0.5mL per cover slip (total of 4 coverslips) or 2mL per flaskette.

3. Incubate cultures undisturbed at 37°C 5%-8% CO2 atmosphere.

4. Flood cultures on day 2 by adding 2mL of CHANG Amnio®.

5. After 4 to 5 days, the cultures should be checked for growth. Cultures should be fed once growth has been observed. Feed cultures by removing all of the culture supernatant and replacing with 2mL fresh CHANG Amnio®. It is recommended that cultures be fed every 2 days thereafter.

6. Check cultures for growth on/or after day 5, and harvest when sufficient colonies are observed.

7. Best results are obtained when the cultures are fed with CHANG Amnio the day before the harvest.

Use of CHANG Amnio for Primary Cultures: Flask Methodologies

原代培养：培养瓶法

1. Centrifuge amniotic fluid at low speed to concentrate the cells.

2. Resuspend the cell pellet in a small volume of the patient’s own amniotic fluid.For example, aspirate the supernatant of 10mL of spun amniotic fluid to 1mL above the cell pellet and resuspend. Add 4mL of CHANG Amnio for a total volume of 5mL per flask.

3. Incubate cultures undisturbed at 37°C 5%-8% CO2 atmosphere.

4. Check for growth on day 5. Change medium with fresh CHANG Amnio and harvest if sufficient cell growth is observed.

5. Check cultures for growth and compley change medium every day thereafter until sufficient colonies are observed and are ready to harvest.

6. Best results are obtained when the cultures are fed with CHANG Amnio the day before the harvest.

Use of CHANG Amnio for Growing Passaged Amniotic Fluid Cells:

传代培养

To passage the cells, treat the cultures with trypsin (or pronase, etc.) as you would normally do when cells are grown in conventional medium. However, protease treatment should be carefully monitored. Amniotic fluid cells grown in CHANG Amnio tend to be more sensitive to protease treatment than amniotic fluid cells grown in conventional medium. It may be necessary to modify your protocol to take this into account.The pH of the medium used to feed the cultures must be between 6.65–7.44 (i.e.the medium must be slightly yellowish salmon color).pH can easily be adjusted by placing the medium in a 5%-8% CO2 incubator with the cap slightly loosened for about 30 minutes.

Note: Calcium Oxalate crystals commonly form in CHANG Amnio. The presence of these crystals has not been shown to cause any detrimental effect on product performance. 

Irvine CHANG Amnio 羊水细胞培养基,完全型现货数量有限 详询 华雅生物集团旗下公司：红荣微再（上海）生物工程技术有限公司  ：1500 1904 520。红荣微再-客服:  经销商专员