G5154 Glasgow Minimum Essential Medium （G...

Glasgow Minimum Essential Medium was originally developed by lan MacPherson and Michael Stoker as a modification of Eagle′s medium. This medium was used to study the genetic factors affecting cell competence. The polyoma virus was used to transform four fibroblast clones from a culture of baby hamster kidney cells. The medium was developed by modifying Eagle′s BME by adding 10% tryptose phosphate (e.g., Sigma Prod. No. T8159 at 100 ml/L medium) and twice the normal concentration of amino acids and vitamins.