免疫球蛋白重链基因位点抗体,Anti-IGH抗体背景介绍:Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. This region represents the germline organization of the heavy chain locus. The locus includes V (variable), D (diversity), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single D segment with a J segment; this partially rearranged D-J gene is then joined to a V segment. The rearranged V-D-J is then transcribed with the IGHM constant region; this transcript encodes a mu heavy chain. Later in development B cells generate V-D-J-Cmu-Cdelta pre-messenger RNA, which is alternatively spliced to encode either a mu or a delta heavy chain. Mature B cells in the lymph nodes undergo switch recombination, so that the V-D-J gene is brought in proximity to one of the IGHG, IGHA, or IGHE genes and each cell expresses either the gamma, alpha, or epsilon heavy chain. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Due to polymorphism, the numbers of functional V, J, and D genes differ among individuals and some V, D, J, and C segments may be pseudogenes.
【产品名称】:免疫球蛋白重链基因位点抗体,Anti-IGH抗体
【产品类型】:一抗
【抗体别名】:IGH; IGH.1@; IGHDY1; Immunoglobulin heavy locus.
【相关标记】: HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,Alexa Fluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor 647标记抗体
博研生物抗体质量保证,为您的实验保驾护航,,是博研生物优势供应产品:,客服
【性 状】: Lyophilized or Liquid
【浓 度】: 1mg/1ml
【亚 型】: IgG
【规 格】:0.1ml/100ug,0.2ml/200ug
【抗体来源】: Rabbit OR MOUSE
【克隆类型】: polyclonal or monoclonal
【产品用途】:科研实验,用于免疫组化实验,WB实验、IF、IP、ELISA实验,相应的标记抗体有HRP标记抗体,FITC标记,BIO等。
【贮 存】: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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操作步骤:
1.切片常规脱蜡至水。如需抗原修复,可在此步后进行
⒉ 缓冲液洗 3min/2 次。
⒊ 为了降低内源性过氧化物酶造成的非特异性背景染色,将切片放在 Hydrogen Peroxide Block 中孵育 10-15 分钟。
⒋ 缓冲液洗 5min/2 次。
⒌ 滴加 Ultra V Block , 在室温下孵育 5 分钟以封闭非特异性的背景染色。
(注:孵育不要超过 10 分钟,否则会导致特异性染色降低。如果一抗的稀释液中含有 5 - 10% 正常羊血清,这一步可以省略。)
⒍ 缓冲液洗 5min/2 次。
⒎ 滴加一抗工作液 37 ℃孵育 1 - 2 小时。(具体孵育时间和温度由试验者Z终决定)
⒏ 缓冲液洗 5min/2 次。
9 . 滴加 Primary Antibody Enhancer(增强子),在室温下孵育 20 分钟。
10 .缓冲液洗 5min/2 次。
11 .滴加 HRP Polymer(酶标二抗) ,在室温下孵育 30 分钟。
(注:HRP Polymer 对光敏感,应避免不必要的光暴露并储存在不透明的小瓶中。)
12 .缓冲液洗 5min/2 次。
13 .向 1ml DAB Plus Substrate (或 AEC Plus Substrate) 中滴加 1-2 滴 DAB Plus Chromogen (或 AEC Plus Chromogen),混匀后滴加到切片上,孵育 3 - 15 分钟。(具体时间由染色深浅决定。)
14 .自来水充分冲洗,复染,脱水,透明,封片。
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