Agilent RNA 6000 Pico Kit 安捷伦(Agilent )5067-1513 RNA 6000 Pico kit
For the analysis of low concentrated RNA samples down to 50 pg/µL of total RNA or 250 pg/µL of mRNA. The kit includes 25 microfluidic chips, reagents and consumables.
RNA Kits & Reagents - Details & Specifications
RNA quality control and quantitation is essential before any downstream application. Both RNA kits offer:
- Quantitation and integrity analysis of total RNA or mRNA
- RNA Integrity Number (RIN) – to compare integrity of total RNA samples
- Visualization of rRNA impurities in mRNA samples
- High sensitivity RNA analysis, e.g. samples derived from microdissection
安捷伦(Agilent )5067-1513 RNA 6000 Pico kit
![](http://item.yiqi.com/pic/ConPic/2/030813_RNAQC.jpg) | RNA QC The electropherograms shows the analysis of high quality total RNA (RIN 9.2) in comparison to a partially degraded total RNA (RIN 5.8). (Application Note 5989-1165EN) |
Analytical specifications | RNA 6000 Nano Kit | RNA 6000 Pico Kit |
total RNA | mRNA | total RNA | mRNA |
Quantitative range | 25 - 500 ng/µL | 25 - 250 ng/µL | - | - |
Qualititative range | 5 - 500 ng/µL | 5 - 250 ng/µL | 50 - 5000 pg/µL in water | 250 - 5000 pg/µL in water |
Sensitivity (S/N>3) | 5 ng/µL in water | 25 ng/µL in water | 50 pg/µL in water or 200 pg/µL in TE | 250 pg/µL in water or 500 pg/µL in TE |
Quantitation reproducibility (within a chip) | 10% CV | 10% CV | 20% CV | 20% CV |
Quantitation accuracy1 | 20% CV | 20% CV | 30% CV | - |
Maximum sample buffer strength | 100 mM Tris 0.1 mM EDTA or 125 mM NaCl 15 mM MgCl2 | 100 mM Tris 0.1 mM EDTA or 125 mM NaCl 15 mM MgCl2 | 50 mM Tris 0.1 mM EDTA or 50 mM NaCl 15 mM MgCl2 | 50 mM Tris 0.1 mM EDTA or 50 mM NaCl 15 mM MgCl2 |
Physical specifications |
Analysis time | 30 minutes | 30 minutes | 30 minutes | 30 minutes |
Samples per chip | 12 | 12 | 11 | 11 |
Sample volume | 1 µL | 1 µL | 1 µL | 1 µL |
Kit stability | =4 months at 4°C | =4 months at 4°C | =4 months at 4°C | =4 months at 4°C |
Kit size | 25 chips
12 samples/chip = 300 samples/kit | 25 chips
12 samples/chip = 300 samples/kit | 25 chips
11 samples/chip = 275 samples/kit | 25 chips
11 samples/chip = 275 samples/kit |
1Determined analyzing the RNA ladder as sample
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供应商:上海恒斐生物科技有限公司 刘鑫