The Neon® Transfection System 100 µL Kit is designed specifically for use with the Neon® Transfection System. Use this kit for transfection volumes of 100 µL, containing 5 × 105–2 × 106 adherent cells or 1 × 106–5 × 106 suspension cells. Cells that have been transfected using the included 100 µL Neon® Tips are then ready to be washed and plated into a 6-well, 60 mm, or 10 cm culture dish, depending on your protocol.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Neon®-transfected mouse hippocampal explants.
Transfected human adipose-derived stem cells.
规格
Format: | Electroporation Pipette Tip |
---|
System: | Neon® Transfection System |
---|
Cell Type: | Established Cell Lines, Hard-to-Transfect Cells, Stem Cells, Primary Cells |
---|
Product Size: | 96 x 2 reactions |
---|
Classification: | Animal Origin-Free, Chemically Defined, Low-Protein, Xeno-Free, Reduced Serum, Reduced Growth Factors |
---|
Serum Compatible: | Yes |
---|
Shipping Condition: | Room Temperature |
---|
Compatible Products: | Transfection Grade Nucleic Acid purification kits, siRNA vectors, DNA vectors |
---|
Sample Loading Volume: | 100µl |
---|
Sample Type (Specific): | Plasmid DNA, Synthetic siRNA |
---|
Transfection Technique: | Electroporation |
---|
For Use With (Equipment): | Neon® Transfection System |
---|
Starting Material (Cell #): | 2x10^4 - 6 x10^8 cells; Varies by cell type |
---|
内容及储存
• 30 ml Resuspension Buffer R
• 30 ml Resuspension Buffer T
• 2 × 150 mL Electrolytic Buffer E2
• 96 × 100 µl Neon® Tips
• 20 Neon® Electroporation Tubes
Store at room temperature. After first use, store buffers at 4°C.