D8020 Deoxyribonucleic acid sodium salt f...

Deoxyribonucleic acid sodium salt from calf thymus
货号：D8020
规格：50mg/支

• 英文名称：Deoxyribonucleic acid sodium salt from calf thymus（DNA(calf thymus)）

• 别名：ctDNA;胸腺核酸钠盐;脱氧核糖核酸钠盐（来源于小牛胸腺）

• CAS：73049-39-5

• 储存条件：2-8°C

• 外观（性状）：纤维状的

• 单位：支

    小牛胸腺DNA(CT-DNA)是一种天然的DNA广泛用于研究DNA绑定剂和DNA绑定代理,调节DNA结构和功能。

    小牛胸腺DNA的DNA也被运用于物理化学研究行为的解决方案。 溶解性：溶于水，参考浓度1mg/ml，4℃条件下轻轻摇晃为佳方法，但不可剧烈震荡。

Preparation Note
This product is extracted using a method that causes shearing yielding a highly polymerized mixture of double and single stranded DNA . However, double stranded DNA is the predominant form.
This product is prepared from calf thymus tissue of unspecified gender.
Quality
"Highly Polymerized"
Reconstitution
Solutions of DNA have been stored successfully for several months at 4 C, pH 7.5-8, in 10 mM Tris with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (μg/ml) DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store DNA in highly alkaline solutions since DNA tends to degrade at alkaline pH greater than 8.0.
This product can be dissolved at 2 mg/ml in water. To reduce further shearing of the DNA, no sonication or stirring should be used. Gentle inversion overnight at 0-4 °C is recommended to compley solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.
Physical form
fibrous preparation
Application
Calf thymus DNA (CT-DNA) is a natural DNA widely used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Calf thymus DNA is also used in physicochemical studies of DNA behavior in solution.
Physical properties
λmax 259 nm (100 mM phosphate buffer, pH 7.0).
DNA from calf thymus is 41.9 mole % G-C and 58.1 mole % A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 μg of double-stranded DNA.

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