NUCLEOSIL® NH2 / NH2 RP 液相色谱柱_详细资料

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供货周期现货

Nucleosil NH2 氨基柱
※      Nucleosil 高性能硅胶，特殊技术键合，使NH2键合相在水相介质中稳定，低流失，保证柱子长寿命※     键合相采用非端基封尾，具有更广泛的选择性※      有100, 120 and 300 Å，三种孔径；有3um, 5um, 7um, 10um 四种粒度
※ pH stability at 20 °C: 2–8, carbon content 3.5 %※      可以使用多种流动相体系，分析不同性质样品：
    * 正相流动相（正乙烷，二氯乙烯，异丙醇），分析苯胺取代物，酯类和有机氯农药
    * 水和有机物作为正相流动相，可以分析极性样品，如：糖类、植物甙类
    * 反相中添加缓冲盐，作为弱离子交换体系可以分析有机酸类，阴离子化合物
NUCLEOSIL® NH2 / NH2 RP
Amino modified silica phases for HPLC · USP L8

Features
Polar aminopropyl modified silica for reversed phase and normal phase chromatography:
Normal phase chromatography with hexane, dichloromethane or 2-propanol as phase for polar compounds such as substituted anilines, esters, chlorinated pesticides
Reversed phase chromatography of polar compounds like carbohydrates in aqueous-organic eluent systems
Anion exchange chromatography of anions and organic acids using common buffers (e.g., acetate or phosphate) in conjunction with organic modifiers (e.g., acetonitrile)
Available pore sizes 100, 120 and 300 Å, available particle sizes 3 µm, 5 µm, 7 µm and 10 µm,
pH stability at 20 °C: 2–8, carbon content 3.5 %
Separation mechanism based on polar interactions, hydrophobic interactions and weak ion exchange interactions
Corresponding NUCLEODUR® phase see NUCLEODUR® NH2 / NH2-RP
For applications click here.

产品应用:
双糖检测
色谱柱：Nucleosil 100-5 NH2, 250×4 mm
流动相：乙腈：水=75:25
流速：1毫升/分钟

样品：1. 甲基葡萄糖苷 2. 果糖 3. 葡萄糖 4. 蔗糖 5. 乳糖 6. 麦芽糖

HPLC检测 Curcumin （姜黄素）及相关组份

色谱柱：Nucleosil 100-5 NH2, 250×4.6 mm ID
流动相：96% 乙醇
流速：1.2毫升/分钟
检测器：fluorescence, excitation 420nm, emission 470nm. The curcumino ids can also be detected by UV-VIS spectroscopy at 254 or 420nm.

样品：1. Bisde methoxycurcumin 2. Demethoxycurcumin 3.Curcumin
发酵液中多糖的检测
色谱柱：Nucleosil 100-5 NH2, 250×4 mm
流动相：乙腈：水=65:35
流速：2毫升/分钟
温度：80℃
样品：1. 葡萄糖 2. 纤维素二糖 3-8.不同聚合度葡聚糖

订货信息:
EC standard columns for analytical HPLC
Ordering information
Eluent in column (except for NUCLEOSIL® 100-5 NH2 RP) is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is necessary to rinse the column with THF first.

ID
Particle size
Pore size

Length 250 mm

EC guard
columns*
CC guard
columns**
NUCLEOSIL® 100-3 NH2 (eluent in column n-heptane)

4.6 mm
3 µm
100 Å

720275.46

721933.30
721122.40
NUCLEOSIL® 100-5 NH2 RP (eluent in column acetonitrile – water)

4.6 mm
5 µm
100 Å

720095.46RP

721155.30
721605.40RP
NUCLEOSIL® 100-5 NH2 (eluent in column n-heptane)

4.6 mm
5 µm
100 Å

720095.46

721020.30
721605.40
NUCLEOSIL® 100-10 NH2 (eluent in column n-heptane)

4.6 mm
10 µm
100 Å

720025.46

Columns/pack ->

1

3
3
* EC 4/3 guard column cartridges are used for EC columns of 4.6 mm ID with the Column Protection System guard column holder (REF 718966).
** CC 8/4 guard column cartridges are used for EC columns of 4.6 mm ID with guard column adapter EC (REF 721359).
Custom-packed EC columns with other dimensions are available on request.

NUCLEOSIL® NH2 bulk packingsOrdering information
Phase

Carbon content
Pore size
Particle size
Pack of 10 g
Pack of 100 g
NUCLEOSIL 100-5 NH2

3.5 %
100 Å
5 µm
712200.10
712200.100
NUCLEOSIL 100-10 NH2

3.5 %
100 Å
10 µm
712210.10
712210.100
NUCLEOSIL 120-7 NH2

~ 2 %
120 Å
7 µm
712610.10
712610.100
NUCLEOSIL 300-7 NH2

~ 2 %
300 Å
7 µm
712919.10
712919.100

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NUCLEOSIL® NH2 / NH2 RP
Amino modified silica phases for HPLC · USP L8

EC standard columns for analytical HPLC
Ordering information
Eluent in column (except for NUCLEOSIL® 100-5 NH2 RP) is n-heptane. When using an eluent which is not miscible with n-heptane (e.g., water), it is necessary to rinse the column with THF first.

ID	Particle size	Pore size		Length 250 mm		EC guard columns*	CC guard columns**
NUCLEOSIL® 100-3 NH2 (eluent in column n-heptane)
4.6 mm	3 µm	100 Å		720275.46		721933.30	721122.40
NUCLEOSIL® 100-5 NH2 RP (eluent in column acetonitrile – water)
4.6 mm	5 µm	100 Å		720095.46RP		721155.30	721605.40RP
NUCLEOSIL® 100-5 NH2 (eluent in column n-heptane)
4.6 mm	5 µm	100 Å		720095.46		721020.30	721605.40
NUCLEOSIL® 100-10 NH2 (eluent in column n-heptane)
4.6 mm	10 µm	100 Å		720025.46
Columns/pack ->				1		3	3
* EC 4/3 guard column cartridges are used for EC columns of 4.6 mm ID with the Column Protection System guard column holder (REF 718966). CC 8/4 guard column cartridges are used for EC columns of 4.6 mm ID with guard column adapter EC (REF 721359)**.
Custom-packed EC columns with other dimensions are available on request.
NUCLEOSIL® NH2 bulk packingsOrdering information
Phase		Carbon content	Pore size	Particle size	Pack of 10 g	Pack of 100 g
NUCLEOSIL 100-5 NH2		3.5 %	100 Å	5 µm	712200.10	712200.100
NUCLEOSIL 100-10 NH2		3.5 %	100 Å	10 µm	712210.10	712210.100
NUCLEOSIL 120-7 NH2		~ 2 %	120 Å	7 µm	712610.10	712610.100
NUCLEOSIL 300-7 NH2		~ 2 %	300 Å	7 µm	712919.10	712919.100