V01031 必拓生物pMAL-c2x载体，pMAL-c2x vector

V01031必拓生物pMAL-c2x载体，pMAL-c2x vector，质粒载体图谱

MBP（麦芽糖结合域）融合表达载体，促进可溶，tac启动子，胞内表达
N-末端MBP融合标签，GX促可溶表达

成分
10 mM Tris（pH8.0）

保存条件
-20℃保存

产品目录

pMAL-c2x载体，pMAL-c2x vector，质粒载体图谱

LOCUS       pMAL-c2X        6646 bp    DNA     circular SYN 22-MAR-2012

DEFINITION  pMAL-c2X.

SOURCE      

  ORGANISM  other sequences; artificial sequences; vectors.

FEATURES             Location/Qualifiers

     source          1..6646

                     /organism="pMAL-c2X"

                     /mol_type="other DNA"

                     /vntifkey="98"

     misc_feature    72..1163

                     /vntifkey="21"

                     /label=lacI

     promoter        1407..1435

                     /vntifkey="30"

                     /label=tac_promoter

     CDS             1528..2916

                     /vntifkey="4"

                     /label=MBP(leaderfree)

     CDS             3418..4278

                     /gene="Ampicillin"

                     /vntifkey="4"

                     /label=Ampicillin

                     /note="ORF frame 1"

     gene            3418..4278

                     /gene="Ampicillin"

                     /vntifkey="60"

     rep_origin      4928..5547

                     /vntifkey="33"

                     /label=pBR322_origin

BASE COUNT     1695 a      1670 c      1767 g      1514 t

ORIGIN

        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga

       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg

      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa

      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac

      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc

      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg

      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc

      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca

      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga

      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc

      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg

      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag

      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga

      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa

      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg

      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc

      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga

     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata

     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt

     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag

     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg

     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg

     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt

     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga

     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga

     1501 gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact ggtaatctgg

     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat

     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt

     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac

     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat

     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt

     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa

     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac

     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag

     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg

     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac

     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg

     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc

     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt

     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg

     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag

     2461 ttggcgaaag atccacgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg

     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc

     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg

     2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat ttcagaattc

     2701 ggatcctcta gagtcgacct gcaggcaagc ttggcactgg ccgtcgtttt acaacgtcgt

     2761 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc

     2821 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg

     2881 aatggcgaat ggcagcttgg ctgttttggc ggatgagata agattttcag cctgatacag

     2941 attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg

     3001 gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt

     3061 gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca

     3121 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag

     3181 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc

     3241 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg

     3301 cctttttgcg tttctacaaa ctctttttgt ttatttttct aaatacattc aaatatgtat

     3361 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg

     3421 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt

     3481 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga

     3541 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa

     3601 gaacgttctc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt

     3661 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt

     3721 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc

     3781 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga

     3841 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat

     3901 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct

     3961 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc

     4021 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg

     4081 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc

     4141 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg

     4201 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca

     4261 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta

     4321 ccccggttga taatcagaaa agccccaaaa acaggaagat tgtataagca aatatttaaa

     4381 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt

     4441 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag

     4501 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg

     4561 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat

     4621 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc

     4681 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga

     4741 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac

     4801 ccgccgcgct taatgcgccg ctacagggcg cgtaaaagga tctaggtgaa gatccttttt

     4861 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc

     4921 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg

     4981 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact

     5041 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg

     5101 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg

     5161 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac

     5221 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca

     5281 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga

     5341 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc

     5401 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct

     5461 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg

     5521 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct

     5581 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc

     5641 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc

     5701 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca

     5761 caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt

     5821 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc

     5881 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac

     5941 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca

     6001 gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc

     6061 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc

     6121 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct

     6181 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga

     6241 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg

     6301 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg

     6361 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg

     6421 cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt

     6481 tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga

     6541 ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag

     6601 cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gaaatt

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