| CBP60533 |
| I. General information |
| Synonyms: | RAW264.7 |
| Background: | This line was established from a tumor induced by Abelson murine leukemia virus. |
| Species: | Mus musculus, mouse |
| Tissue: | Abelson murine leukemia virus-induced tumor; ascites |
| Disease: | Abelson murine leukemia virus-induced tumor |
| Gender: | male,adult |
| Morphology: | monocyte/macrophage |
| Growth Mode: | adherent |
| DNA Profile: | Cobioer’s Cell Line Authentication Service |
| Culture Medium: | DMEM+10%FBS |
| We strongly suggest to purchase the complete medium from Cobioer. |
| Cryopreservation medium: | Complete growth medium supplemented with 5% (v/v) DMSO |
| Antigen Expression: | H-2d |
| Receptor Expression: | complement (C3) Ref |
| Genes Expressed: | lysozyme, H-2d,Tested and found negative for ectromelia virus (mousepox). |
| Cellular Products: | lysozyme |
| Comments: | This cell line is easy to propagate, high efficiency for DNA transfection, sensitivity to RNA interference, and supports replication of murine noroviruses. This cell line is negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2). When this line was established, it was described as not secreting detectable virus particles and negative using the XC plaque formation assay. Based on a published study by Dr. Janet W. Hartley in 2008, this line was demonstrated to express ecotropic and polytropic MuLV, and is positive using the XC plaque assay for virus replication.Ref |
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| For more information, please contact Cobioer (4008-750-250). |
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