PeproTech 大血管内皮细胞培养基 PeproGrow 700-...

英文名：PeproGrow™ MacroV (Macrovascular Endothelial Cell Media)

中文名：PeproGrow™ MacroV 大血管内皮细胞培养基

PeproGrow MacroV 大血管内皮细胞培养基适用于培养大血管内皮细胞。能良好维持内皮祖细胞的形态和功能，并提高特定标志物—内皮一氧化氮合酶活性。

概述

PeproGrow™ MacroV 大血管内皮细胞培养基

Product Details

Catalog Number:700-MacroV

Description:

PeproTech offers three separate endothelial cell culture media formulations developed for the in vitro cultivation of: endothelial progenitor cells (EPC; PeproGrow EPC) derived from bone marrow or peripheral blood; endothelial cells from large vessels (PeproGrow MacroV); and endothelial cells from small vessels (PeproGrow MicroV). These media formulations maintain outstanding endothelial cell morphology and function, and increase the activity of endothelial nitric oxide synthase (eNOS), which account for a specific, crucial marker for endothelial cells. By doing this, the media provide an optimal cell culture environment for macrovascular and microvascular endothelial cells, as well as for EPC; growing cells at rates that exceed commercially available media. These media formulations are optimized for primary/progenitor human cells, but can also be used for bovine, porcine, rabbit, rat, and mouse EPC, or mature endothelial cells. 

PeproGrow MacroV is supplied as a 500mL bottle of basal medium and a separate, frozen growth factor component.

PeproGrow MacroV内皮细胞培养基适用细胞：
PeproGrow MacroV is recommended for Macrovascular Endothelial Cells:
Human Umbilical Vein Endothelial Cells (HUVEC) 人脐静脉内皮细胞
Human Umbilical Artery Endothelial Cells (HUAEC) 人脐动脉内皮细胞
Human Aortic Endothelial Cells (HAoEC) 人大动脉内皮细胞
Human Pulmonary Artery Endothelial Cells (HPAEC) 人肺动脉内皮细胞
Human Saphenous Vein Endothelial Cells (HSaVEC) 人隐静脉内皮细胞
And other large vessel (macrovascular) endothelial cells 以及其他大血管内皮细胞

PeproGrow MacroV内皮细胞培养基用于人脐静脉内皮细胞扩增的性能

Figure 1: HUVEC Proliferation. HUVEC were seeded onto fibronectin-coated plates and incubated for 7 days in PeproGrow MacroV basal medium + Growth Supplement MacroV and two competitors' medium. The proliferation ability of HUVEC was assessed 7 days after cell cultivation using the XTT assay following the manufacturer’s protocol. The proliferation ability of HUVEC was expressed as the average of fluorescence intensity (O.D.) calculated using a plate reader from two independent assays run in triplicates.
 人脐静脉内皮细胞扩增

PeproTech 内皮细胞培养基系列

PeproTech提供三种内皮细胞培养基试剂盒，分别适用于体外培养：或外周血来源的内皮祖细胞（PeproGrow™ EPC ），大血管内皮细胞（PeproGrow™ MacroV）和微血管内皮细胞（PeproGrow™ MicroV）。每款试剂盒由基础培养基和一管单独的内皮细胞生长添加剂组成。

其他内皮细胞培养基

ScienCell 1001 内皮细胞培养基 现货供应

欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  ：1500 1904 520。红荣微再-客服:  经销商专员

背景——人脐静脉内皮细胞

来源：Wiki

Human umbilical vein endothelial cells (HUVECs) are cells derived from the endothelium of veins from the umbilical cord. They are used as a laboratory model system for the study of the function and pathology of endothelial cells (e.g., angiogenesis).[1] They are used due to their low cost, and simple techniques for isolating them from umbilical cords, which are normally resected after childbirth. HUVECs were first isolated and cultured in vitro in the 1970s by Jaffe and others. [2] HUVECs can be easily made to proliferate in a laboratory setting. Like human umbilical artery endothelial cells they exhibit a cobblestone phenotype when lining vessel walls.

人脐静脉内皮细胞来自脐带血管的内皮细胞。因为价格低和分离技术简单，人脐静脉内皮细胞通常作为内皮细胞的功能和生理研究的实验模型使用。人脐静脉内皮细胞是在1970年由Jaffe等人率先分离使用的。在实验条件下人脐静脉内皮细胞很容易扩增。

人脐静脉内皮细胞（HUVEC）获取

来源：互联网

1. 将 15-20cm 长的新生儿脐带放入无菌的 PBS 溶液中储存。（注：4℃下Z多贮存 24 小时，室温下不超过 6 小时，否则废弃）

2. 用一个钝头的针头扎入脐带静脉管中，用无菌的 PBS 溶液冲洗 3-5 次，将污血冲洗干净为止。

3. 用手术钳夹紧脐带下端，加入 15ml 的胶原酶（1mg/ml）室温下消化 15-20分钟，并不时上下摇动脐带。

4. 消化完后，将下端手术钳松开，消化液流入一个 50 ml 无菌离心管中，用无菌的 PBS 溶液冲洗脐带 2-3 次。

5. 将收集液离心（2000 转/ 分）3 分钟。

6. 倒去上清。加培养基。

欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  ：1500 1904 520。红荣微再-客服:  经销商专员