德国MN色谱柱NUCLEOSIL® Chiral-1/2/3..._详细资料

产品信息

供货周期现货

产品特点:

NUCLEOSIL® CHIRAL-1
NUCLEOSIL® CHIRAL-2
NUCLEOSIL® CHIRAL-3
Silica-based phases for HPLC enantiomer separation
Features

NUCLEOSIL® CHIRAL-1
for enantiomer separation based on ligand exchange · USP L32
Base material NUCLEOSIL® silica, particle size 5 μm, pore size 120 Å,
chiral selector L-hydroxyproline – Cu2+ complexes
Principal interaction mode:
formation of ternary mixed-ligand complexes with Cu(II) ions
Differences in the stability of the diastereomeric complexes cause chromatographic separation.
Eluent in column 0.5 mM copper sulfate solution
Recommended application: enantiomers with two polar functional groups with the correct spacing such as α-amino acids, α-hydroxycarboxylic acids (e.g.,lactic acid), N-alkyl-α-amino acids etc.
NUCLEOSIL® CHIRAL-2 / NUCLEOSIL® CHIRAL-3
for enantiomer separation in organic eluent systems · USP L36
Base material NUCLEOSIL® silica, particle size 5 μm, pore size 100 Å,
chiral selector for NUCLEOSIL® CHIRAL-2: N-(3,5-dinitrobenzoyl)-D-phenylglycine, for NUCLEOSIL®CHIRAL-3 the optical antipode of the CHIRAL-2 phase, "brush type" phases
Principle interaction modes:
charge-transfer interactions, hydrogen bonds, dipole-dipole interactions and steric effects
Eluent in column n-heptane – 2-propanol – TFAA (100:0.05:0.05)
Recommended application: analysis of stereoisomers such as separation of enantiomers and diastereomers, control of optical purity of plant protectives (pesticides, e.g., propionic acid derived herbicides), pharmaceuticals etc. and for product control in chiral organic syntheses

For control of the optical purity of a substance, the two columns NUCLEOSIL® CHIRAL-2 and NUCLEOSIL® CHIRAL-3 allow to select conditions such that the minor enantiomer, which is present as an impurity, is eluted before the main peak. Thus, overlapping peaks are avoided. This makes an exact quantification of the impurity much easier.
For more applications click here.

NUCLEOSIL Chiral-1配位交换手性柱
   配位交换色谱LEC(ligand exchange chromatography)的分离原理是，被测手性样品与色谱柱的官能团中的金属阳离子（CU2+）产生三重混合配位交换，而L-羟基-脯氨酸产生的不对称ZX导致了对样品的手性色谱拆分。NUCLEOSIL CHIRAL-1 配位交换手性柱色谱条件的选择我们建议使用CUSO4的水溶液作为NUCLEOSIL CHIRAL-1的流动相，并可以按一下原则通过改变流动相条件改善分离效果。
Chiral-2/3刷型交换手性柱
   NUCLEOSIL CHIRAL-2/3手性柱是正相原理分离手性化合物的。它是在Nucleosil硅球上交联键合N-(3,5-二硝基苯酰)-“D,L”-苯基甘氨酸作为手性官能团，分离的原理是刷型交换（brush type）。这种分离原理主要用来控制农药或药物的生产工艺中间体的光学纯度。
   由于NUCLEOSIL CHIRAL-2使用的官能团是D-苯基甘氨酸，而NUCLEOSIL CHIRAL-3使用的官能团是L-苯基甘氨酸，使两种手性柱在分离同一手性化合物时，出峰顺序正好相反。这在质检控制产品光学纯度时是十分重要的，我们可以选择NUCLEOSIL CHIRAL-2或CHIRAL-3，让杂质在主要成份前出峰，避免由于脱尾造成定量的不准确。色谱柱使用条件：
   NUCLEOSIL CHIRAL-2/3手性柱使用的流动相一般是非极性有机溶剂（正乙烷，异辛烷），添加极性有机溶剂（四氢呋喃，醇类，氯代烃）和极少量强酸（三fuyisuan）。强酸通常能够明显的改善手性样品的分离。而被测的碱性样品，需要通过苯甲酰氯或3,5-二硝基苯甲酰氯衍生化后进行检测。
产品应用:
NUCLEOSIL Chiral-1典型应用
如果样品中有两个合适空间位置的极性基团，能够与铜离子产生整合作用，CHIRAL-1就应该能够成功地进行手性拆分。例如α-氨基酸（氨基和羧基），α-羟基羧酸（乳酸等），N-烃基α-氨基酸，以及小分子量的手性化合物。D,L-phenylalanine, D,L-pyenylglycine, D,L-proline, D,L-threonine, D,L-valine.

CU2+浓度

增加CU2+浓度可以缩短样品的保留时间。典型的CU2+浓度应该在0.2m M-3m M之间；如下图NUCLEOSIL CHIRAL-1在分离（D,L）-methionine蛋氨酸时，CU2+浓度的提高，分离度相应下降。
有机改性剂
加入有机溶剂（如甲醇：乙腈）可以缩短样品的保留时间，提高出峰对称性。
温度
提高温度可以相应提高柱效，缩短样品的保留时间，建议一般使用温度60℃，Z高的柱压不超过140bar。如下图NUCLEOSIL CHIRAL-1在分离D,L-alanine丙氨酸时，较低的温度，可以提高样品的分离度。
NUCLEOSIL CHIRAL-2键合相
   N-(3,5-二硝基苯酰)-D-苯基甘氨酸化学键合Nucleosil球形硅胶，5um保存流动液；正乙烷：异丙醇：三fuyisuan=100:0.5:0.5
NUCLEOSIL CHIRAL-3键合相
   N-(3,5-二硝基苯酰)-D-苯基甘氨酸化学键合Nucleosil球形硅胶，5um保存流动液；正乙烷：异丙醇：三fuyisuan=100:0.5:0.5
D,L-supidimide的手性拆分

色谱柱：NUCLEOSIL CHIRAL-2 250×4mm
流动相：四氢呋喃：正辛烷=10:3
流速：1.0毫升/分钟
检测器：UV 220nm

订货信息:
Ordering information

Length 250 mm

EC guard columns*
CC guard column**
EC analytical columns

NUCLEOSIL® CHIRAL-1

4 mm ID

720081.40 (1/pack)

721188.30 (1/pack)
721455.40 (1/pack)
NUCLEOSIL® CHIRAL-2

4 mm ID

720088.40 (1/pack)

721190.30 (1/pack)
721458.40 (3/pack)
NUCLEOSIL® CHIRAL-3

4 mm ID

720350.40 (1/pack)

721190.30 (1/pack)
721458.40 (3/pack)

* EC 4/3 guard column cartridges are used for EC columns of 4 mm ID with the Column Protection System guard column holder (REF 718966).
** CC 8/4 guard column cartridges are used for EC columns of 4 mm ID with guard column adapter EC (REF 721359).
Guard column cartridges for NUCLEOSIL® CHIRAL-2 and CHIRAL-3 are identical.

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NUCLEOSIL® CHIRAL-1 NUCLEOSIL® CHIRAL-2 NUCLEOSIL® CHIRAL-3
Silica-based phases for HPLC enantiomer separation
Features

	NUCLEOSIL® CHIRAL-1 for enantiomer separation based on ligand exchange · USP L32 Base material NUCLEOSIL® silica, particle size 5 μm, pore size 120 Å, chiral selector L-hydroxyproline – Cu2+ complexes Principal interaction mode: formation of ternary mixed-ligand complexes with Cu(II) ions Differences in the stability of the diastereomeric complexes cause chromatographic separation. Eluent in column 0.5 mM copper sulfate solution Recommended application: enantiomers with two polar functional groups with the correct spacing such as α-amino acids, α-hydroxycarboxylic acids (e.g.,lactic acid), N-alkyl-α-amino acids etc.
	NUCLEOSIL® CHIRAL-2 / NUCLEOSIL® CHIRAL-3 for enantiomer separation in organic eluent systems · USP L36 Base material NUCLEOSIL® silica, particle size 5 μm, pore size 100 Å, chiral selector for NUCLEOSIL® CHIRAL-2: N-(3,5-dinitrobenzoyl)-D-phenylglycine, for NUCLEOSIL®CHIRAL-3 the optical antipode of the CHIRAL-2 phase, "brush type" phases Principle interaction modes: charge-transfer interactions, hydrogen bonds, dipole-dipole interactions and steric effects Eluent in column n-heptane – 2-propanol – TFAA (100:0.05:0.05) Recommended application: analysis of stereoisomers such as separation of enantiomers and diastereomers, control of optical purity of plant protectives (pesticides, e.g., propionic acid derived herbicides), pharmaceuticals etc. and for product control in chiral organic syntheses
	For control of the optical purity of a substance, the two columns NUCLEOSIL® CHIRAL-2 and NUCLEOSIL® CHIRAL-3 allow to select conditions such that the minor enantiomer, which is present as an impurity, is eluted before the main peak. Thus, overlapping peaks are avoided. This makes an exact quantification of the impurity much easier.
	For more applications click here.

Ordering information
	Length 250 mm	EC guard columns*	CC guard column**
EC analytical columns
NUCLEOSIL® CHIRAL-1
4 mm ID	720081.40 (1/pack)	721188.30 (1/pack)	721455.40 (1/pack)
NUCLEOSIL® CHIRAL-2
4 mm ID	720088.40 (1/pack)	721190.30 (1/pack)	721458.40 (3/pack)
NUCLEOSIL® CHIRAL-3
4 mm ID	720350.40 (1/pack)	721190.30 (1/pack)	721458.40 (3/pack)

* EC 4/3 guard column cartridges are used for EC columns of 4 mm ID with the Column Protection System guard column holder (REF 718966). CC 8/4 guard column cartridges are used for EC columns of 4 mm ID with guard column adapter EC (REF 721359)**. Guard column cartridges for NUCLEOSIL® CHIRAL-2 and CHIRAL-3 are identical.