TANFO02KIT_T TwistDx核酸检测试剂TwistAmp nfo TANFO02KI..._详细资料

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Basic Information:RPA1) Primers must be 30-35 bases2) Works best at constant temperature (37-39°C)3) Amplicons of 80-400bp are preferred4) TwistAmp? LF Probe or TwistAmp? exo Probe 1 required - see overleafSet-up (single-plex)21) Prepare reaction mix in 1.5ml tube: Primer A (10μM) 2.1 μl Primer B (10μM) 2.1 μl TwistAmp? LF Probe (10μM) 0.6 μl Rehydration Buffer 29.5 μl Template and dH2O 13.2 μl (Total Volume 47.5 μl) Vortex and spin briefly2) Add reaction mix to freeze-dried reaction. Pipette to mix.3) Add 2.5 μl of 280mM MgAc (supplied) and mix well to start reaction.4) Incubate at 37-39°C for 20-40 minutes.4b) For low template copy number,remove strip after 4 minutes, vortex &spin briefly, replace in heating device. 5) For analysis by lateral flow, dilute reacion products 1/50 with PBST and load 10 μl onto the sample pad of appropriate strip (eg Milenia? HybriDetect strips).6) Place strip in PBST running buffer.WARNING: RPA REACTIONS START AT ROOM TEMPERATURE AS SOON AS MAGNESIUM IS ADDED.

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TANFO02KIT_T TwistDx核酸检测试剂TwistAmp nfo TANFO02KI...

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