T4 DNA 聚合酶
PRODUCT DESCRIPTION产品描述
T4 DNA Polymerase catalyzes the extension of a primed DNA template in the 5′→ 3′ direction. This enzyme exhibits a powerful 3′→ 5′ exonuclease activity, while lacking any inherent 5′→ 3′ exonuclease or strand displacement functions.
Enzymatics T4 DNA 聚合酶 T4 DNA Polymerase P7080L
Source of Protein蛋白来源
Purified from a strain of E. coli that expresses the recombinant T4 DNA Polymerase gene.
从能够表达重组T4 DNA聚合酶基因的大肠杆菌菌株中纯化得来
Supplied in
100 mM KPO4
1.0 mM DTT
0.1 mM EDTA
50% glycerol
pH 6.5 @ 25°C
Supplied With
B0110 (10X Blue Buffer)
10X Blue Buffer (B0110)
500 mM NaCl
100 mM Tris-HCl
100 mM MgCl2
10 mM DTT
pH 7.9 @ 25°C
Unit Definition单位定义
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-precipitable material in 30 minutes at 37°C.
1 unit 被定义为在37°C下、30分钟内能够将10 nmol dNTP转化成酸性不溶物的酶量
PRODUCT INFORMATION产品信息
T4 DNA Polymerase
Part Number P7080L
Concentration 3,000 U/ml
Unit Size 2,000 U
SDS Available on request
PRODUCT SPECIFICATION*产品特性
Storage Temperature -15 to -25°C
Test Specification
Purity (SDS-PAGE) >99%
Specific Activity 5,555 U/mg
SS Exonuclease Functional
DS Exonuclease Functional
DS Endonuclease 30 U = no conversion
E.coli DNA Contamination 30 U < 10 copies
* For a detailed summary of assay conditions and data, refer to the Quality Controls Analysis section.
REFERENCES参考文献
Tabor, S. and Struhl, K. (1989) In DNA-Dependent DNA Polymerases. F. M. Ausebel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith and K. Struhl (Eds.), Current Protocols in Molecular Biology, pp. 3.5.10-3.5.12. 2. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, (2nd Ed.), 5.44-5.47.
Dale, R., McClure, B. and Houchins, J. (1985) Plasmid, 13, 31-40.
Kunkel, T.A., Roberts, J.D. and Zakour, R.A. (1987) R. Wu and L. Grossman (Eds.), Methods Enzymol., 154, pp. 367-382. San Diego: Academic Press.
Panet, A., van de Sande, J.H., Loewen, P.C. and Khorana, H.G. (1973) Biochemistry, 12, 5045-5050.
Limitations of Use适用范围
该产品被研制、生产加工及销售,仅用于试管内,不适用于人类和动物