NSE 神经元特异性烯醇化酶（NSE）单克隆抗体

HZA537Hu22 Monoclonal Antibody to Enolase, Neuron Specific (NSE) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 11th Edition (Revised in May, 2016) [ PROPERTIES ] Source: Monoclonal antibody preparation Host: Mouse Antibody isotype: IgG2b Kappa Purification: Protein A/G Affinity Chromatography. Clone number: C20 Traits: Liquid Concentration: 500µg/mL UOM: 200µg Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. 神经元特异性烯醇化酶(NSE)单克隆抗体[ IMMUNOGEN ] Immunogen: RPA537Hu02-Recombinant Enolase, Neuron Specific (NSE) [ APPLICATIONS ] Western blotting: 0.5-5ug/ml Immunocytochemistry in formalin fixed cells: 5-30ug/ml Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml Immunohistochemistry in paraffin section: 5-30ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. [ FORMULATION ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant NSE disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine (DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). 神经元特异性烯醇化酶(NSE)单克隆抗体Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 4 oC for frequent use. Aliquot and store at -20 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition. 神经元特异性烯醇化酶(NSE)单克隆抗体[ IDENTIFICATION ] A Figure 1. Western Blot A. Sample: Recombinant NSE, Human B. Lane1: Human Hela Cells Lane2: Human Hepg2 Cells Primary Ab: 2µg/mL Rabbit Anti-Human NSE Ab Second Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab (Catalog: SAA544Mu09) B Figure2. DAB staining on IHC-P Samples： A. Human Brain Tissue B. Human Liver Tissue C. Human Glioma Tissue D. Human Stomach Tissue A B C D神经元特异性烯醇化酶(NSE)单克隆抗体HZ998Mu01    磷脂酰肌醇蛋白聚糖4(GPC4)重组蛋白        Recombinant Glypican 4 (GPC4)    Mus musculus (Mouse)
HZ000Hu01    白细胞关联免疫球蛋白样受体2(LAIR2)重组蛋白        Recombinant Leukocyte Associated Immunoglobulin Like Receptor 2 (LAIR2)    Homo sapiens (Human)
HZ008Hu01    低密度脂蛋白受体(LDLR)重组蛋白        Recombinant Low Density Lipoprotein Receptor (LDLR)    Homo sapiens (Human)
HZ009Ra01    极低密度脂蛋白受体(VLDLR)重组蛋白        Recombinant Very Low Density Lipoprotein Receptor (VLDLR)    Rattus norvegicus (Rat)
HZ010Hu01    低密度脂蛋白受体相关蛋白1(LRP1)重组蛋白        Recombinant Low Density Lipoprotein Receptor Related Protein 1 (LRP1)    Homo sapiens (Human)
HZ010Mu01    低密度脂蛋白受体相关蛋白1(LRP1)重组蛋白        Recombinant Low Density Lipoprotein Receptor Related Protein 1 (LRP1)    Mus musculus (Mouse)
HZ011Hu01    Runt相关转录因子2(RUNX2)重组蛋白        Recombinant Runt Related Transcription Factor 2 (RUNX2)    Homo sapiens (Human)
HZ011Mu01    Runt相关转录因子2(RUNX2)重组蛋白        Recombinant Runt Related Transcription Factor 2 (RUNX2)    Mus musculus (Mouse)
HZ014Hu01    整合素关联蛋白(IAP)重组蛋白        Recombinant Integrin Associated Protein (IAP)    Homo sapiens (Human)
HZ015Hu01    α1-抗胰糜蛋白酶(α1ACT)重组蛋白        Recombinant Alpha-1-Antichymotrypsin (a1ACT)    Homo sapiens (Human)
HZ017Hu01    α2-巨球蛋白(α2M)重组蛋白        Recombinant Alpha-2-Macroglobulin (a2M)    Homo sapiens (Human)
HZ017Mu01    α2-巨球蛋白(α2M)重组蛋白        Recombinant Alpha-2-Macroglobulin (a2M)    Mus musculus (Mouse)
HZ017Ra01    α2-巨球蛋白(α2M)重组蛋白        Recombinant Alpha-2-Macroglobulin (a2M)    Rattus norvegicus (Rat)
HZ018Bo01    α-乳清蛋白(αLA)重组蛋白        Recombinant Alpha-Lactalbumin (aLA)    Bos taurus; Bovine (Cattle)
HZ018Hu01    α-乳清蛋白(αLA)重组蛋白        Recombinant Alpha-Lactalbumin (aLA)    Homo sapiens (Human)
HZ019Hu01    血管生成素样蛋白4(ANGPTL4)重组蛋白        Recombinant Angiopoietin Like Protein 4 (ANGPTL4)    Homo sapiens (Human)