IFNg 干扰素γ（IFNg）活性蛋白

HZA049Mu01 100µg Active Interferon Gamma (IFNg) Organism Species: Mus musculus (Mouse) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 1th Edition (Apr, 2016) [ PROPERTIES ] Source: Prokaryotic expression. Host: E. coli Residues: His23~Cys155 Tags: N-terminal His-tag Purity: >95% Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). 干扰素γ(IFNg)活性蛋白Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and Proclin300. Predicted isoelectric point: 8.8 Predicted Molecular Mass: 16.8kDa Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions. Applications: Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.) [ USAGE ] Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. [ STORAGE AND STABILITY ] 干扰素γ(IFNg)活性蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition. [ SEQUENCE ] [ ACTIVITY ] IFN-γ is an important activator of macrophages, it promotes production of inducible Nitric Oxide Synthase (iNOS) in macrophages. After stimulated with IFN-γ, morphological changes will occur in murine macrophage cell line (Raw 246.7 cells), and inducible nitric-oxide synthase (iNOS) in the cells will increase. Raw 246.7 cells were incubated in DMEM with IFN-γ (2ng/mL) for 24h, then cells were observed by inverted microscope and iNOS in cell lysates was detected by ELISA. Effect of IFN-γ on morphological change of Raw 246.7 cells is shown in Figure 1. Figure 1. Morphological change of Raw 246.7cells after stimulation of IFNg. (A) Raw 264.7 cells cultured in DMEM, stimulated with IFNg;干扰素γ(IFNg)活性蛋白 (B) Unstimulated Raw 246.7 cells cultured in DMEM (negative control). A B Effect of IFN-γ on the expression of iNOS is shown in Table 1. Table 1. ELISA detection of iNOS expression from RAW 246.7 cells stimulated by IFNg Sample (cell lysates of Raw 246.7 cells) Concentration of iNOS (ng/mL) Stimulated with IFNg (2ng/mL) 16.25 Unstimulated 2.71 [ IDENTIFICATION ] 干扰素γ(IFNg)活性蛋白

HZ160Hu01    α2-纤溶酶YZ因子(α2PI)重组蛋白        Recombinant Alpha-2-Plasmin Inhibitor (a2PI)    Homo sapiens 

(Human)
HZ160Mu01    α2-纤溶酶YZ因子(α2PI)重组蛋白        Recombinant Alpha-2-Plasmin Inhibitor (a2PI)    Mus musculus 

(Mouse)
HZ160Ra01    α2-纤溶酶YZ因子(α2PI)重组蛋白        Recombinant Alpha-2-Plasmin Inhibitor (a2PI)    Rattus 

norvegicus (Rat)
HZ160Rb01    α2-纤溶酶YZ因子(α2PI)重组蛋白        Recombinant Alpha-2-Plasmin Inhibitor (a2PI)    Oryctolagus 

cuniculus (Rabbit)
HZ162Bo01    嗅素4(OLFM4)重组蛋白        Recombinant Olfactomedin 4 (OLFM4)    Bos taurus; Bovine (Cattle)
HZ162Hu01    嗅素4(OLFM4)重组蛋白        Recombinant Olfactomedin 4 (OLFM4)    Homo sapiens (Human)
HZ162Mu01    嗅素4(OLFM4)重组蛋白        Recombinant Olfactomedin 4 (OLFM4)    Mus musculus (Mouse)
HZ164Ga01    泛素(Ub)重组蛋白        Recombinant Ubiquitin (Ub)    Chicken (Gallus)
HZ164Gu01    泛素(Ub)重组蛋白        Recombinant Ubiquitin (Ub)    Cavia (Guinea pig )
HZ164Si01    泛素(Ub)重组蛋白        Recombinant Ubiquitin (Ub)    Rhesus monkey (Simian)
HZ165Bo01    胃蛋白酶原A(PGA)重组蛋白        Recombinant Pepsinogen A (PGA)    Bos taurus; Bovine (Cattle)
HZ165Hu01    胃蛋白酶原A(PGA)重组蛋白        Recombinant Pepsinogen A (PGA)    Homo sapiens (Human)
HZ165Ra01    胃蛋白酶原A(PGA)重组蛋白        Recombinant Pepsinogen A (PGA)    Rattus norvegicus (Rat)
HZ166Ga01    胃蛋白酶原C(PGC)重组蛋白        Recombinant Pepsinogen C (PGC)    Chicken (Gallus)
HZ166Hu01    胃蛋白酶原C(PGC)重组蛋白        Recombinant Pepsinogen C (PGC)    Homo sapiens (Human)
HZ166Mu01    胃蛋白酶原C(PGC)重组蛋白        Recombinant Pepsinogen C (PGC)    Mus musculus (Mouse)
HZ166Ra01    胃蛋白酶原C(PGC)重组蛋白        Recombinant Pepsinogen C (PGC)    Rattus norvegicus (Rat)
HZ167Hu01    前列腺素E合酶(PTGES)重组蛋白        Recombinant Prostaglandin E Synthase, Microsomal (PTGES)    Homo 

sapiens (Human)
HZ168Hu01    前列腺酸性磷酸酶(ACP3)重组蛋白        Recombinant Acid Phosphatase 3, Prostatic (ACP3)    Homo sapiens 

(Human)