IL10 白介素10（IL10）活性蛋白

HZA056Hu61 100µg Active Interleukin 10 (IL10) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 1th Edition (Apr, 2016) [ PROPERTIES ] Source: Eukaryotic expression. Host: 293F cell Residues: Ser19~Asn178 Tags: N-terminal His-tag Purity: >98% Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and Proclin300. Predicted isoelectric point: 7.6 Predicted Molecular Mass: 白介素10(IL10)活性蛋白20.3kDa Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions. Applications: Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.) [ USAGE ] Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 oC for 48h, 白介素10(IL10)活性蛋白and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition. [ SEQUENCE ] [ ACTIVITY ] Interleukin 10 (IL-10), also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. IL-10 was initially reported to suppress cytokine secretion, antigen presentation and CD4 + T cell activation. Further investigation has shown that IL-10 predominantly inhibits lipopolysaccharide (LPS) mediated induction of the pro-inflammatory cytokines TNFα, IL-1β, IL-12, and IFNγ made by cells such as macrophages and Th1 T cells. Therefore, THP1 cells were cultured in 24 well plates at a concentration of 10 6 cells/ml and activated by LPS (1ug/mL) in the absence or presence of IL-10 (5ng/mL, 10ng/mL) for 24h and production of IL-1β was determined in the supernatants by cytokine specific ELISA. 白介素10(IL10)活性蛋白Activation of THP1 cells with LPS (1ug/mL) resulted in high levels of production of IL-1β and strong inhibitory effects of IL-10 (5ng/mL, 10ng/mL) were observed on the production of IL-1β as shown in Table 1. Table 1. Inhibitory effects of IL-10 on IL-1β production by THP1 cells [ IDENTIFICATION ] Figure 1. SDS-PAGE Sample: Active recombinant IL10, Human Figure 2. Western Blot Sample (cell supernatant of THP1 cells) 白介素10(IL10)活性蛋白

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Homo sapiens (Human)
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Mus musculus (Mouse)
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Homo sapiens (Human)