神经生长因子(NGF)真核蛋白HZA105Hu61 100ug Eukaryotic Nerve Growth Factor (NGF) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 11th Edition (Revised in May, 2016) [ PROPERTIES ] Source: Eukaryotic expression. Host: 293F cell Residues: Glu19~Arg239 Tags: N-terminal His Tag Homology: Mouse 85%, rat 86% Tissue Specificity: Serum. Subcellular Location: 神经生长因子(NGF)真核蛋白Secreted. Purity: >95% Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Traits: Freeze-dried powder Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL Predicted isoelectric point: 9.9 Predicted Molecular Mass: 26.3kDa Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions. Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays;神经生长因子(NGF)真核蛋白 Purification; Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.) Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: 神经生长因子(NGF)真核蛋白Dimerization, multimerization etc. [ USAGE ] Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 oC for one month. Aliquot and store at -80 oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition. 神经生长因子(NGF)真核蛋白
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