葡萄糖磷酸变位酶3(PGM3)重组蛋白RPE743Hu01 100ug Recombinant Phosphoglucomutase 3 (PGM3) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PROPERTIES ] Residues: Leu12~Leu245 Tags: Two N-terminal Tags, His-tag and T7-tag Accession: O95394 Host: E. coli Purity: >95% Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl,葡萄糖磷酸变位酶3(PGM3)重组蛋白 pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. Predicted isoelectric point: 6.3 Predicted Molecular Mass: 29.6kDa Accurate Molecular Mass: 15&33kDa as determined by SDS-PAGE reducing conditions. Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.) Note: The possible reasons that the actual band size differs from the predicted are as follows: 1. 葡萄糖磷酸变位酶3(PGM3)重组蛋白Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. 葡萄糖磷酸变位酶3(PGM3)重组蛋白[ USAGE ] Reconstitute in sterile ddH2O. [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) 葡萄糖磷酸变位酶3(PGM3)重组蛋白The loss of this protein is less than 5% within the expiration date under appropriate storage condition. [ SEQUENCES ] The sequence of the target protein is listed below. LHAKPNGLIL QYGTAGFRTK AEHLDHVMFR MGLLAVLRS KQTKSTIGVM VTASHNPEED NGVKLVDPLG EMLAPSWEEH ATCLANAEEQ DMQRVLIDI SEKEAVNL DAFVVIGRDT RPSSEKLSQS VIDGVTVLGG QFHDYGLLT TPQLHYMVYC RNTGGRYGKA TIEGYYQKLS KAFVELTKQA SCSGDEYRS LKVDCANGIG ALKLREMEHY 葡萄糖磷酸变位酶3(PGM3)重组蛋白FSQGLSVQLF NDGSKGKL
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