HIPK4 同源域相互作用蛋白激酶4（HIPK4）重组蛋白

同源域相互作用蛋白激酶4(HIPK4)重组蛋白RPF475Hu01 100ug Recombinant Homeodomain Interacting Protein Kinase 4 (HIPK4) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 10th Edition (Revised in Jan, 2014) [ PROPERTIES ] Residues: Leu288~His616 Tags: Two N-terminal Tags, His-tag and T7-tag Accession: Q8NE63 Host: E. coli Subcellular Location: Cytoplasm.同源域相互作用蛋白激酶4(HIPK4)重组蛋白 Purity: >90% Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. Predicted isoelectric point: 5.4 Predicted Molecular Mass: 39.8kDa Accurate Molecular Mass: 44kDa as determined by SDS-PAGE reducing conditions. Applications: SDS-PAGE; WB; ELISA; IP. (May be suitable for use in other assays to be determined by the end user.) Note: The possible reasons that the actual band size differs from the predicted are as follows: 1.同源域相互作用蛋白激酶4(HIPK4)重组蛋白 Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc. [ USAGE ] Reconstitute in sterile ddH2O. [ STORAGE AND STABILITY ] Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. Stability Test: The thermal stability is described by the loss rate of the target protein. 同源域相互作用蛋白激酶4(HIPK4)重组蛋白The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition. [ SEQUENCES ] The sequence of the target protein is listed below.同源域相互作用蛋白激酶4(HIPK4)重组蛋白 LDQ IETV NGGSVA SR LTFPDREA LAE HA DLKSM VELIKRM LTW ESHE RISPSA ALRHPFVSMLRSAHETTH YYQLSLRSYR LSLQVEGKPP TPVVAAEDGT PYYCLAEEKE AA GMGS VA GS SP F F REE K A P GMQ RAI D Q L D DL S LQE A G H G LW GE T C T NAV SDMMVPLKAA ITGHHVPDSG PEPILAFYSS RLAGRHKARK PPAGSKSDSN FSNLIRLSQV SPEDDRPCRG SSWEEGEHLG ASAEPLAILQ RDEDGPNIDN MTMEAERPDP 同源域相互作用蛋白激酶4(HIPK4)重组蛋白ELFDPSSCPG EWLSEPDCTL ESVRGPRAQG LPPRRSHQHG PPRGATSFLQ HVTGHH

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