Anti-AIF抗体,调亡诱导因子抗体,芳香烃受体抗体

Anti-AIF抗体,调亡诱导因子抗体优质供应-上海研晶生物专业为您提供，欢迎咨询说明书等详细信息
来源：rabbit mouse
规格：0.1ml/0.2ml
产品类型 ： 一抗  多抗；   产品价格：电询；    保存： Store at -20 °
：Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase 9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed.
( Not yet tested in other applications.Optimal dilutions/concentrations should be determined by the end user)
Anti-AIF抗体,调亡诱导因子抗体现货0.1ml；0.2ml，制备一般包括以下几个步骤：
 1、制备抗原。 　
2、选择实验动物。
3、动物免疫。 　
4、试取血进行测试，看看是否成功免疫。
5、如果成功免疫，杀死实验动物，采集全部血清。
6、纯化出抗体。 　
7、鉴定抗体。包括纯度以及特异性
抗原修FF法:
化学方法
加热方法
水浴加热法
微波照射法
高压加热法
酸水解法
一抗是针对抗原的抗体，二抗是针对一抗的抗体。即抗体也可以充当抗原刺激机体产生抗体。
一抗体就是我们平时所说的抗体，它能和抗原特异性结合。
第二抗体是能和抗体结合，即抗体的抗体，其主要作用是检测抗体的存在。
蛋白质与多肽的联系：
多肽：通常由10~100氨基酸分子脱水缩合而成的化合物叫多肽，它们的分子量低于10，000Da(Dalton，道尔顿)，能透过半透膜，不被三氯酸及硫铵所沉淀。也有文献把由2~10个氨基酸组成的肽称为寡肽(小分子肽)；10~50个氨基酸组成的肽称为多肽；由50个以上的氨基酸组成的肽就称为蛋白质。
Anti-AIF抗体,调亡诱导因子抗体属我公司优质产品，部分相关产品如下：
咪唑 0527-10G 288-32-4 10G 生物技术级 Yes 4 years
咪唑 0527-50G 288-32-4 50G 生物技术级 Yes 4 years
 0527-1KG  1KG   
结晶紫 0528-100G 548-62-9 100G 美国药典级 No 4 years
结晶紫 0528-500G 548-62-9 500G 美国药典级 No 4 years
柠檬酸 0529-1KG 77-92-9 1KG ACS级 No 4 years
柠檬酸 0529-3KG 77-92-9 3KG ACS级 No 4 years
柠檬酸 0529-500G 77-92-9 500G ACS级 No 4 years
醋酸钠,三水 0530-1KG 6131-90-4 1KG ACS级 No 4 years
醋酸钠,三水 0530-2.5KG 6131-90-4 2.5KG ACS级 No 4 years
醋酸钠,三水 0530-500G 6131-90-4 500G ACS级 No 4 years
溴甲酚紫 0531-100G 115-40-2 100G 试剂级 No 4 years
溴甲酚紫 0531-25G 115-40-2 25G 试剂级 No RS-10479B 人丙型肝炎IgM(HCV-IgM)试剂盒秋季促销，96T/48T免费代测 Human Hepatitis C virus IgM,HCV-IgM Elisa试剂盒
RS-10480B 人丙型肝炎IgG(HCV-IgG)试剂盒秋季促销，96T/48T免费代测 Human Hepatitis C virus IgG,HCV-IgG Elisa试剂盒
RS-10481B 人单胺氧化酶(MAO)试剂盒秋季促销，96T/48T免费代测 Human monoamine oxidase,MAO Elisa试剂盒
RS-10482B 人抗流行性出血热病毒抗体IgM (EHF)试剂盒秋季促销，96T/48T免费代测  Human anti-epidemic hemorrhagic fever virus IgM antibody,EHF IgM Elisa试剂盒
RS-10483B 人抗狂犬病毒抗体(anti-RV)试剂盒秋季促销，96T/48T免费代测  Human anti-rabies virus antibody, anti-RV Elisa试剂盒
RS-10484B 人抗副流感病毒IgM抗体(anti-PIV IgM)试剂盒秋季促销，96T/48T免费代测  Human anti-parainfluenza virus IgM antibody, anti-PIV IgM Elisa试剂盒
RS-10485B 人抗风疹病毒IgG抗体(anti-RV IgG)试剂盒秋季促销，96T/48T免费代测 Human anti-rubella virus IgG antibody,anti-RV IgG Elisa试剂盒
RS-10486B 人抗斑疹伤寒抗体(anti-typhus-Ab)试剂盒秋季促销，96T/48T免费代测  Human anti-typhus antibody Elisa试剂盒
RS-10487B 人抗EB病毒抗体(EBV)试剂盒秋季促销，96T/48T免费代测  Human anti-Epstein-Barr virus antibody,EBV Elisa试剂盒
RS-10488B 人肺炎支原体抗体(MP-Ab)试剂盒秋季促销，96T/48T免费代测  Human Mycoplasma pneumoniae antibody,MP-Ab Elisa试剂盒
RS-10489B 人肺炎衣原体抗体(Cpn-Ab)试剂盒秋季促销，96T/48T免费代测  Human Chlamydia pneumoniae antibody,Cpn-Ab Elisa试剂盒
RS-10490B 人单核细胞增多性李斯特菌素(listeriolysin)试剂盒秋季促销，96T/48T免费代测  Human listeriolysin Elisa试剂盒
RS-10491B 人单纯疱疹病毒Ⅱ型抗体(HSVⅡ-Ab)试剂盒秋季促销，96T/48T免费代测 Human Herpes simplexvirus Ⅱ antibody,HSVⅡ-Ab Elisa试剂盒
RS-10492B 人腺相关病毒(AAV)试剂盒秋季促销，96T/48T免费代测 Human adeno-associated virus,AAV Elisa试剂盒
RS-10493B 人皮肤反应因子/炎性因子(SRF/IF)试剂盒秋季促销，96T/48T免费代测  Human skin reactive factor/inflammatory factor,SRF/IF Elisa试剂盒
RS-10494B 人细菌性阴道病(BV)试剂盒秋季促销，96T/48T免费代测  Human bacterial vaginosis,BV Elisa试剂盒
Anti-AIF抗体,调亡诱导因子抗体进口优质抗体，现货目录供应，低价促销ing,公司销售各种类抗体，产品数量多，产品齐全，抗体纯化、抗体制备，公司权威提供技术支持。提供单克隆抗体制备、多克隆抗体制备、抗体标记服务。   
技术文章：
多克隆抗体：
定义1：由多个B细胞克隆所产生的抗体，可与不同抗原表位结合且免疫球蛋白类别各异。
所属学科：免疫学(一级学科)；免疫系统(二级学科)；免疫分子(三级学科)
定义2：对特定抗原所产生的一组免疫球蛋白混合物，每种免疫球蛋白能识别抗原分子上的一个表位。
所属学科：生物化学与分子生物学(一级学科)；总论(二级学科)
定义3：多种抗原表位刺激机体免疫系统后，机体产生的针对不同抗原表位的混合抗体。
所属学科：细胞生物学(一级学科)；细胞免疫(二级学科)
ab1998 staining AIF in human U2OS cells by Immunocytochemistry/ Immunofluorescence.
Samples were fixed using 4% paraformaldehydeICC/IF image of ab1998 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab1998, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.