| ibidi Standard Bottom | ibidi Glass Bottom |
Optical properties |
Refractive index (nD 589 nm) | 1.52 | 1.52 |
Abbe number | 56 | 55 |
Thickness | #1.5 (180 μm) | #1.5 (170 μm) |
Material | Microscopy plastic | D 263M Schott borosilicate glass |
Autofluorescence | Low | Low |
Transmission | Very high (even ultraviolet) | High (ultraviolet restrictions) |
Birefringence (DIC) | Low (DIC compatible*) | Low (DIC compatible*) |
Other aspects |
Surface modifications | ibiTreat – tissue culture treated Uncoated – hydrophobic | Only pure glass |
Protein coatings | Possible | Possible |
Gas permeable | Yes | No |
Material flexibility | High | Low |
Breakable | No | Yes |
Recommended for | All kinds of fluorescence microscopy | TIRF and single photon |