Hep G2人肝癌细胞系,Hep G2细胞_详细资料

产品信息

细胞名称
Hep G2人肝癌细胞
描述
该细胞系来自15岁男性白人的组织。形态为上皮形，模式染色体数为55，在免疫YZ小鼠中不致瘤。
动物种别
人
性别
男
组织来源
肝细胞癌
形态
上皮细胞
培养基和添加剂
MEM培养基(GIBCO,添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制
仅供研究之用。
REFERENCE
Knowles BB , et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960 Knowles BB , Aden DP . Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983 Schardt C , et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141 Aden DP , et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137 Busch SJ , et al. Differential regulation of hepatic triglyceride lipase and 3-hydroxy-3- methylglutaryl-CoA reductase gene expression in a human hepatoma cell line, HepG2. J. Biol. Chem. 265: 22474-22479, 1990. PubMed: 2176219 Darlington GJ , et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851 Cuthbert C , et al. Regulation of human apolipoprotein A-I gene expression by gramoxone. J. Biol. Chem. 272: 14954-14960, 1997. PubMed: 9169468 Deleersnyder V , et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed: 8985401 Benn J , et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004 Goodrum FD , et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Kolanus W , et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Lewis W , et al. Fialuridine and its metabolites inhibit DNA polymerase gamma at sites of multiple adjacent analog incorporation, decrease mtDNA abundance, and cause mitochondrial structural defects in cultured hepatoblasts. Proc. Natl. Acad. Sci. USA 93: 3592-3597, 1996. PubMed: 8622980 Jang SI , et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Roesler WJ , et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491 Lee JH , et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Lieber A , et al. Recombinant adenoviruses with large deletions geneerated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J. Virol. 70: 8944-8960, 1996. PubMed: 8971024 Dubuisson J , Rice CM . Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J. Virol. 70: 778-786, 1996. PubMed: 8551615 Yamaguchi Y , et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055 Kounas MZ , et al. Cellular internalization and degradation of antithrombin III-thrombin, heparin cofactor II-thrombin, and alpha1-antitrypsin-trypsin complexes is mediated by the low density lipoprotein receptor-related protein. J. Biol. Chem. 271: 6523-6529, 1996. PubMed: 8626456 Klemm DJ , et al. Adenovirus E1A proteins regulate phosphoenolpyruvate carboxykinase gene transcription through multiple mechanisms. J. Biol. Chem. 271: 8082-8088, 1996. PubMed: 8626493 Wu X , et al. Demonstration of a physical interaction between microsomal triglyceride transfer protein and apolipoprotein B during the assembly of ApoB-containing lipoproteins. J. Biol. Chem. 271: 10277-10281, 1996. PubMed: 8626595 Ostlund RE Jr, et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564 Cancer Research 63:3775-3782,2003 Chinese Journal of Cancer 21(3):267-271, 2002 Chin J Dig. 22(6):331-334,2002 Chin J Cancer Biother 9(3):175-178,2002 Natl Med J China 82(8):564-567,2002 Chinese Journal of Cancer 22(3):248-252,2003 Chinese Journal of Cancer 22(12):1280-1283,2003 The Practical Journal of Cancer 18(5):490-491,2003 Immunological Journal 19(3):208-210,2003 J Fujian Med Univ 37(2):162-165,2003 Shanghai Immunol Journal 23(1):16-19,2003
中文名称
人肝癌细胞

Hep G2人肝癌细胞系,Hep G2细胞
我们细胞的特点：
1. 细胞直接来源于ATCC、ECACC、DSMZ、JCRB、KCLB等，公司海外直接购买，未通过代理；
2. 进口Gibco试剂培养；
3. >5年细胞培养经验的硕士负责培养；
4. QC检测：每个细胞发货前都必须经过QC检测，确保每个批次的质量；
5. 细胞质保：收到细胞的1个月内出现任何非人为操作的细胞质量问题，均可免费再次提供；
6. 质保周期长：1个月，给客户充分的时间，开展细胞状态判断和细胞鉴定工作；
7. 杜绝假细胞：支持客户鉴定STR或者基因突变测序数据，有假包退，合同约束；
8. 售前、售后全程免费。
http://www./st315944

质量保证:
我们的细胞株来源于ATCC、ECACC、DSMZ、JCRB等，购买到货后，由我们实验室扩增、冻存，冻存的产品全部经过QC检测，进口来源，保证5代以内，活力>95%，无细菌、真菌、支原体污染。
细胞到达客户手中，1个月内出现任何问题，导致细胞在冻存前死亡，我们公司都可以免费再向客户提供一次。
对细胞的真实性，支持客户鉴定STR或者基因突变测序数据，如证明细胞错误，全额退款。
细胞培养相关问题：1、CO2 培养箱之水盘如何保持清洁？
定期（至少每两周一次）以无菌蒸馏水或无菌去离子水更换之。
2、为何培养基保存于4 ℃ 冰箱中，颜色会偏暗红色，且pH值会越来越偏碱性？
培养基保存于4 ℃ 冰箱中，培养基内之CO2 会逐渐溢出，造成培养基越来越偏碱性。而培养基中之酸碱指示剂（通常为phenol red）的颜色也会随碱性增加而更偏暗红。培养基偏碱之结果，将造成细胞生长停滞或死亡。若培养基偏碱时，可以通入无菌过滤之CO2，以调整pH 值。
3、各种细胞培养用的dish，flask是否均相同？
不同厂Pai的dish 或flask，其所coating 的polymer 不同，制造程序亦不同，虽对大部分细胞没有太大之影响，惟少数细胞则可能因使用厂Pai不同之dish 或flask 而有显著之生长差异。
4、购买之细胞冷冻管经解冻后，为何会发生细胞数目太少之情形？购买之细胞死亡或细胞存活率不佳可能原因？
研究人员在细胞培养时出现存活率不佳，常见原因可归纳为：培养基使用错误或培养基品质不佳。血清使用错误或血清的品质不佳。解冻过程错误。冷冻细胞解冻后，加以洗涤细胞和离心。悬浮细胞误认为死细胞。培养温度使用错误。细胞置于–80℃太久。

【中文名称】：Hep G2人肝癌细胞系,Hep G2细胞
【细胞形式】：复苏好活的细胞
【质量控制】：本细胞经过了检测，不含有细菌、真菌、病毒（HIV、HBV、HCV）、支原体。
【培养条件】：37℃，5% CO2，PH值7.2~7.4，无菌恒温培养。
【研究范围】：本产品只提供给进一步的科研使用，不可应用于ZL等其他方面。
细胞研究的发展：随着细胞生物学尤其是干细胞研究的快速发展，在细胞ZL领域已经进行了大量的探索，取得了一系列的令人振奋的成果，且部分基础研究成果已经在心血管系统疾病、神经系统疾病、肌肉骨骼相关疾病、糖尿病等的临床试验中得到应用，向人们展示了其广阔的应用前景。然而，细胞ZL作为一种新型的人类疾病ZL手段，确切的作用机制还不清晰，临床应用的有效性和安全性依然是目前科学家关注和争论的焦点。
上海研盟生物科技有限公司，在广大科研用户的帮助和支持下，经过多年不懈的努力，已成为国内生命科学试剂的国际运营商之一，代理许多国际先进水平的高科技产品，
依托复旦大学，集研发、销售、实验室技术服务于一体的高科技企业。上海研盟生物专业经营进口科研抗体、细胞、ELISA试剂盒等实验室试剂、化工产品、实验室消耗品、实验室耗材以及实验室器材。产品涉及分子生物学、细胞生物学、细菌学、遗传学、免疫学、生物化学、蛋白质学、细胞ZL、仪器仪表、临床应用等领域。

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细胞名称	Hep G2人肝癌细胞
描述	该细胞系来自15岁男性白人的组织。形态为上皮形，模式染色体数为55，在免疫YZ小鼠中不致瘤。
动物种别	人
性别	男
组织来源	肝细胞癌
形态	上皮细胞
培养基和添加剂	MEM培养基(GIBCO,添加NaHCO3 1.5g/L，Sodium Pyruvate 0.11g/L)，90%；优质胎牛血清，10%。气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
供应限制	仅供研究之用。
REFERENCE	Knowles BB , et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960 Knowles BB , Aden DP . Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983 Schardt C , et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141 Aden DP , et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137 Busch SJ , et al. Differential regulation of hepatic triglyceride lipase and 3-hydroxy-3- methylglutaryl-CoA reductase gene expression in a human hepatoma cell line, HepG2. J. Biol. Chem. 265: 22474-22479, 1990. PubMed: 2176219 Darlington GJ , et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851 Cuthbert C , et al. Regulation of human apolipoprotein A-I gene expression by gramoxone. J. Biol. Chem. 272: 14954-14960, 1997. PubMed: 9169468 Deleersnyder V , et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed: 8985401 Benn J , et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004 Goodrum FD , et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Kolanus W , et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Lewis W , et al. Fialuridine and its metabolites inhibit DNA polymerase gamma at sites of multiple adjacent analog incorporation, decrease mtDNA abundance, and cause mitochondrial structural defects in cultured hepatoblasts. Proc. Natl. Acad. Sci. USA 93: 3592-3597, 1996. PubMed: 8622980 Jang SI , et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Roesler WJ , et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491 Lee JH , et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Lieber A , et al. Recombinant adenoviruses with large deletions geneerated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J. Virol. 70: 8944-8960, 1996. PubMed: 8971024 Dubuisson J , Rice CM . Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J. Virol. 70: 778-786, 1996. PubMed: 8551615 Yamaguchi Y , et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055 Kounas MZ , et al. Cellular internalization and degradation of antithrombin III-thrombin, heparin cofactor II-thrombin, and alpha1-antitrypsin-trypsin complexes is mediated by the low density lipoprotein receptor-related protein. J. Biol. Chem. 271: 6523-6529, 1996. PubMed: 8626456 Klemm DJ , et al. Adenovirus E1A proteins regulate phosphoenolpyruvate carboxykinase gene transcription through multiple mechanisms. J. Biol. Chem. 271: 8082-8088, 1996. PubMed: 8626493 Wu X , et al. Demonstration of a physical interaction between microsomal triglyceride transfer protein and apolipoprotein B during the assembly of ApoB-containing lipoproteins. J. Biol. Chem. 271: 10277-10281, 1996. PubMed: 8626595 Ostlund RE Jr, et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564 Cancer Research 63:3775-3782,2003 Chinese Journal of Cancer 21(3):267-271, 2002 Chin J Dig. 22(6):331-334,2002 Chin J Cancer Biother 9(3):175-178,2002 Natl Med J China 82(8):564-567,2002 Chinese Journal of Cancer 22(3):248-252,2003 Chinese Journal of Cancer 22(12):1280-1283,2003 The Practical Journal of Cancer 18(5):490-491,2003 Immunological Journal 19(3):208-210,2003 J Fujian Med Univ 37(2):162-165,2003 Shanghai Immunol Journal 23(1):16-19,2003
中文名称	人肝癌细胞