2D电泳样品溶解液（缓冲液）

2D电泳样品溶解液（缓冲液）

英文名字：2D-Xtract?

包装规格：50ml

质量标准：BR

商品货号：MB0536

储存条件： 18~25°C

2D电泳样品溶解液（缓冲液）

特点：

Convenient and simple to use extraction buffer. No preparation required, simply hydrate and use. Prevents urea induced protein carbamylation. Prevents waste of unused reagents. No artifactual protein bands due to dust and human skin contamination. Suitable for sample extraction and solubilization for 2D gel electrophoresis and other applications and rehydration of IPG Strips.

说明：

A suitable protein solubilization buffer for 2D analysis must solubilize proteins effectively, without disturbing the native charge of the proteins. Urea based solubilization buffers solubilize proteins effectively, however can modify the native charge of the proteins, due to carbamylation. Urea exists in equilibrium with ammonium cyanate that modifies α- and ε-amino groups, inducing changes in the isoelectric point of proteins leading to artifactual results. One way to minimize the risk of carbamylation is to prepare the urea based reagents fresh before each use. G-Biosciences developed 2D-Xtract?, a dry urea based pre-mixed and ready-to-use solubilization buffer. Simply add an appropriate volume of the supplied rehydration buffer to the dry buffer and then use to solubilize proteins, saving time and improving the quality of IEF/2D gel electrophoresis (Patent Pending). 2D-Xtract? is also designed to be used as a rehydration buffer for IPG strips. 2D-Xtract? is based on our successful line of FOCUS? Extraction Buffers. During the development of the FOCUS? Extraction Buffers, optimal concentrations of critical agents, buffering and stabilizing agents, including urea, thiourea, CHAPS, and non-detergent sulfobetaines were demonstrated to give optimal protein extraction and improved spot resolution for 2D gel analysis. 2D-Xtract? has optimized concentrations of urea, thiourea, CHAPS and non detergent sulfobetaine (ND SB201).

用途及描述：科研试剂，蛋白电泳与染色

注意：我司产品为非无菌包装，若用于细胞培养，请提前做预处理，除去热原细菌，否则会导致染菌。以上数据仅供参考交流之用。

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