074197 美国戴安Dionex ProPac HIC Protein and P..._详细资料

产品信息

美国戴安Dionex ProPac HIC Protein and Peptide Column
Hydrophobic Interaction Chromatography Columns
for the Separation of Large Biomolecules
Hydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.
Based on 5 ?m ultrahigh purity spherical silica gel particles, with 300 ? pores
Proteins separated under non-denaturing conditions
High protein loading capacity for protein purification applications
Proprietary multidentate silica bonding for enhanced hydrolytic stability
High-resolution HPLC separation of proteins and peptides
Wide range of applications
The ProPac^® HIC-10 column is a high-resolution, high-capacity, silica-based HIC column that provides greater hydrolytic stability under the highly aqueous conditions used in HIC. Examples include the separation of bovine serum proteins, snake venom proteins, enzymes, human skeletal muscle protein (HSMP), monoclonal antibodies, pancreatin, and thrombin and peptide applications including tryptic digests of proteins.

ProPac HIC Columns Specifications
Protein Loading Capacity 340 mg Lysozyme per 7.8 x 75 mm column
Starting Material Ultrapure Spherical Silica
Particle Size 5 ?m
Pressure Limit 4,000 psi
Column Construction Stainless Steel
Phase HIC
Average Pore Diameter 300 ?
Endcapped No
Phase Compatibility 2 M Ammonium sulfate/ phosphate salts, organic solvents for cleanup
pH range pH 2.5 – 7.5
Temperature Limit
< 40 °C
Analytical Columns
ProPac HIC-10 Column (2.1 x 100 mm) 063653
ProPac HIC-10 Column (4.6 x 100 mm) 063655
ProPac HIC-10 (4.6 x 250mm) 074197
ProPac HIC-10 Column (7.8 x 75 mm) 063665

ProPac HIC Columns Specifications
Protein Loading Capacity	340 mg Lysozyme per 7.8 x 75 mm column
Starting Material	Ultrapure Spherical Silica
Particle Size	5 ?m
Pressure Limit	4,000 psi
Column Construction	Stainless Steel
Phase	HIC
Average Pore Diameter	300 ?
Endcapped	No
Phase Compatibility	2 M Ammonium sulfate/ phosphate salts, organic solvents for cleanup
pH range	pH 2.5 – 7.5
Temperature Limit	< 40 °C

Analytical Columns
ProPac HIC-10 Column (2.1 x 100 mm)	063653
ProPac HIC-10 Column (4.6 x 100 mm)	063655
ProPac HIC-10 (4.6 x 250mm)	074197
ProPac HIC-10 Column (7.8 x 75 mm)	063665

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