人胃癌耐药顺铂细胞株 SGC-7901/DDP细胞
形态特性: 成纤维细胞样
生长特性: 贴壁生长
培养条件: M-199: with Earle's Balanced Salts Solution (EBSS) 15%NBS
传代方法: 1:2传代;3-4天一次
品 质:高活性,人胃癌耐药顺铂细胞株 SGC-7901/DDP细胞实验效果好
使用范围:仅供科研使用
冻存条件:基础培养基+5%DMSO+20%FBS
保存与运输:冻存
公司细胞广泛用于国内院校的细胞生物学研究,他们的很多论文都或多或少使用我们公司的细胞,作为实验项目研究。
常见的问题解决方案:
1、培养瓶有破裂,培养液有漏液:细胞极大可能会污染,所以我们会及时安排帮老师解决。
2、细胞漂浮:培养瓶不开封,瓶口酒精擦拭后平躺放置在培养箱。次日观察,如细胞大部分又贴回瓶底,表明细胞活力正常,剩余漂浮的细胞可以去掉,留10ml培养液培养观察,细胞生长至汇合度80%,进行消化传代;如细胞还是不贴壁,将细胞离心收集转到新培养瓶,原培养瓶加部分培养液继续培养,中间注意观察,我们的技术人员会一直跟踪指导,直到问题解决。
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