CRL-1897 UACC 812 人乳腺导管瘤细胞

CRL-1897 UACC 812 人乳腺导管瘤细胞 的详细介绍
CRL-1897 UACC 812 人乳腺导管瘤细胞

ATCC® Number:	CRL-1897™	Price:	$407.00
Designations: UACC-812 Depositors: A Liebovitz, J Trent Biosafety Level: 1 Shipped: frozen Medium & Serum: See Propagation Growth Properties: adherent Organism: Homo sapiens (human) Morphology: epithelial Source: Organ: mammary gland; breast Disease: ductal carcinoma Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. CRL-1897 UACC 812 人乳腺导管瘤细胞 Restrictions: The breast carcinoma UACC-812 was deposited in the ATCC by Albert Leibovitz and Jeffrey M. Trent, Arizona Cancer Center, University of Arizona, Tucson, Arizona. The cells are distributed for research purposes only. The University of Arizona has released the line subject to the following: 1. UACC-812 or their products MUST NOT BE DISTRIBUTED to third parties. Commercial interests are the exclusive property of the University of Arizona. 2. Any proposed commercial use of these cells must first be negotiated with the Director of the Arizona Cancer Center, University of Arizona, 1501 N. Campbell Avenue, Tucson, Arizona 85724. Telephone (602) 626-7925, FAX (602) 626-2284. 3. In all papers reporting any use of these of these cells or derived products, a direct reference will be made to the original publication given above. Isolation: Isolation date: 1986 Receptors: estrogen receptor progesterone receptor Cytogenetic Analysis: range = 58 to 64; abnormal banding region in 3p Age: 43 years Gender: female Comments: This cell line was established by A. Liebovitz and associates in 1986 from breast tissue removed at mastectomy to remove a ductal carcinoma (stage II, grade IV). Prior to surgery, the patient had received extensive chemotherapy. The cells exhibit a 15 fold amplification of the HER-2/neu oncogene sequence. They are negative for estrogen receptors, progesterone receptors and P glycoprotein. Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 2 mM L-glutamine supplemented with 20 ng/ml human EGF and 20% fetal bovine serum Atmosphere: air, Temperature: 37.0°C Growth conditions: The cells grow very slowly, and growth is enhanced by using 20% fetal bovine serum and adding epidermal growth factor (20 ng/ml) to the medium. Subculturing: Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels. Incubate cultures at 37°C. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended Medium Renewal: Every 2 to 3 days Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase Doubling Time: 100 hrs Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008 recommended serum:ATCC 30-2020 purified DNA:ATCC 45534 purified DNA:ATCC 45535 purified DNA:ATCC CRL-1897D References: 22207: . . Proc. Am. Assoc. Cancer Res. 29: 24, 1988. 26165: Meltzer P, et al. Establishment of two new cell lines derived from human breast carcinomas with HER-2/neu amplification. Br. J. Cancer 63: 727-735, 1991. PubMed: 1674877

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iec-6 大鼠小肠隐窝上皮细胞
马-达二氏犬肾细胞系 MDCK (NBL-2) CRL-34
鸭子胚胎成纤维细胞  Duck embryo  CCL-141
小鼠胚胎成纤维细胞 3T3-L1 CCL-173
人结肠直肠腺癌 COLO 320DM CCL-220
人结肠直肠腺癌 NCI-H508 CRL-253
人结肠直肠腺癌 COLO 320HSR CCL-220.1
人B淋巴细胞 Ramos（RA1） CRL-1596
人淋巴母细胞 T2(174×CEM.T2) CRL-1992
大鼠心肌细胞 H9C2(2-1) CRL-1446
CRL-1897 UACC 812 人乳腺导管瘤细胞
猪鼻甲黏膜成纤维细胞 PT-K75 CRL-2528
小鼠癌细胞 LTPA CRL-2389
大鼠癌细胞 DSL-6A/C1 CRL-2132
小鼠瘤B淋巴细胞 SP2/MIL-6 CRL-2016
大鼠脑间质细胞 DI TNC1 CRL-2005
小鼠肌原细胞 C2C12 CRL-1772
鹌鹑纤维肉瘤细胞 QT6 CRL-1708
大鼠回肠细胞 IEC-18 CRL-1589
非洲绿猴肾细胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586
人成纤维细胞 Hs 738.St/Int CRL-7869
人正常前列腺上皮细胞 WPE-int CRL-2888
人正常前列腺成肌纤维细胞 WPMY-1 CRL-2854
人导管腺癌细胞 PL45 CRL-2558
人癌细胞 Panc 05.04 CRL-2557
人癌细胞 Panc 04.03 CRL-2555
人癌细胞 Panc 02.03 CRL-2553
人癌细胞 Panc 08.13 CRL-2551
人癌细胞 Panc 03.27 CRL-2549
人癌细胞 Panc 10.05 CRL-2547
人肝癌细胞 SNU-398 CRL-2233
人成神经瘤细胞- SK-N-DZ CRL-2149
人癌细胞 HPAC CRL-2119
人癌细胞 HPAF-II CRL-1997
人黑色素瘤细胞 A375.S2 CRL-1872
人脐静脉内皮细胞 HUV-EC-C CRL-1730
人黑素瘤细胞 WM-266-4 CRL-1676
人膀胱癌细胞 HT-1376 CRL-1472
人正常前列腺上皮细胞 RWPE-2 CRL-11610
人正常前列腺上皮细胞 RWPE-1 CRL-11609
鸡胚胎成纤维细胞 UMNSAH/DF-1 CRL-12203
人乳腺癌细胞 MDA-MB-231 HTB-26
CRL-1897 UACC 812 人乳腺导管瘤细胞
小鼠巨噬细胞 RAW 264.7 TIB-71
人横纹肌肉瘤细胞 A-204 HTB-82
人癌细胞 Capan-2 HTB-80
人癌细胞 Capan-1 HTB-79
人乳腺导管癌细胞 MDA-MB-435S HTB-129
人胃癌细胞 Hs 746T HTB-135
人肺腺癌细胞 NCI-H441 [H441] HTB-174
人瘤细胞 U266 TIB-196
人结肠癌细胞 NCI-H716 [H716] CCL-251
人多形性恶性胶质瘤 T98G [T98-G] CRL-1690
人神经母细胞瘤细胞 BE(2)-C CRL-2268
人肾透明细胞癌细胞 Caki-2 HTB-47
人皮肤成纤维细胞 Hs 865.Sk CRL-7601
CA46 人淋巴瘤细胞系
CRL-5826 NCI-H226 人肺鳞癌细胞系
Tca-8113 人舌鳞癌细胞
MA-891 小鼠乳腺癌高转移细胞
U14 小鼠宫颈癌细胞
CRL-9518 L5178Y 小鼠淋巴瘤细胞
HTB-112 HEC-1-A 人子宫内膜腺癌细胞
HTB-113 HEC-1-B 人子宫内膜腺癌细胞
AN3 CA 人子宫内膜腺癌细胞
ROS1728 大鼠成骨细胞
CRL-6323 B16F1 小鼠黑色素瘤细胞
HTB-58 SK-MES-1 人肺鳞癌细胞
CCL-127 IMR-32 人神经母细胞瘤细胞
HTB-79 Capan-2 人癌细胞
BALL-1 人B淋巴细胞急性白血病细胞
CRL-1897 UACC 812 人乳腺导管瘤细胞
CRL-2922 Eayh926 人脐静脉细胞融合细胞
WB-F344 大鼠肝干细胞
CRL-1715 TM4 正常小鼠睾丸细胞
CRL-2539 4T1 小鼠乳腺癌细胞
CRL-1442 BRL-3A 大鼠肝细胞
BRL 大鼠肝细胞
RSC96大鼠雪旺细胞
Isk 人子宫内膜癌细胞
HTB-113 hec-1-b 人子宫内膜腺癌细胞
PLA-802?人恶性横纹肌肉瘤
Pt-K2 袋鼠肾细胞
BS-C-1 非洲绿猴肾细胞
A498人肾癌细胞
3AO人卵巢癌细胞
iec-6 大鼠小肠隐窝上皮细胞
u87MG 人脑星形胶质母细胞瘤
Renca 小鼠肾癌细胞
nci-h929 瘤细胞
P388小鼠白血病细胞
CRL-1897 UACC 812 人乳腺导管瘤细胞
opm-2 瘤细胞
MDA-MB-435 人乳腺癌高转移细胞
KU812 人外周血嗜碱性白细胞
NIH:OVCAR-3 人卵巢癌细胞
SW 1990人癌细胞系
S180人结肠腺癌细胞
HuTu-80十二指肠腺癌
RL95-2 人子宫内腺癌细胞
NCI-H446 人小细胞肺癌细胞
MSTO-211H 人肺癌细胞
H4-Ⅱe 大鼠肝癌细胞系
HLF 人肺成纤维样细胞
LS 180人结肠腺癌细胞
HuTu-80十一指肠腺癌
ST猪睾丸细胞系
REH人急性非B非T淋巴细胞白血病
NCI-H520 人肺鳞癌细胞
RKO 人结肠腺癌细胞
293A 人胚肾细胞
RK-13 兔肾细胞系