ProPac IMAC Columns Specifications |
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Maximum Operating Pressure | <3000 psi (21 MPa) |
Temperature Range | <60oC Recommended Temperature: Controlled ambient |
Typical Operating Conditions | <600 psi at 1.0 mL/min (4 × 250mm) Recommended flow rate: 1.0 mL/min |
Phase Compatibility | pH 2–12, Compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl |
Resin Compostion | 10 μm diameter nonporous polystyrene divinylbenzene substrate (55% crosslinking) with poly(IDA) grafts. |
Metal Ion Capacity (μmol Cu) | >80 mmol Cu (4 × 250 mm analytical column) |
Chelating Metals | Ni, Fe, Cu, others |
Analytical Column Dimensions | 1 × 50 mm, 2 × 50 mm, 4 × 50 mm, 9 × 50 mm 2 × 250 mm, 4 × 250 mm, 9 × 250 mm, 22 × 250 mm |
Loading Column Dimensions | 4 × 50 mm, 9 × 50 mm, 9 × 250 mm |