PTPRC抗体

PTPRC抗体

抗原
Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)
别名 PTPRC, si:dkeyp-47c5.1, B220, CD45, CD45R, GP180, L-CA, LCA, LY5, T200, Cd45, Ly-5, Lyt-4, loc, Lca, RT7, cd45
适用
其他选择 Chicken
Human(684),Murine (519),Rat ((Rattus)) (142),Cat (56),Bovine (36),Dog (Canine) (32),Chicken (28),Pig (Porcine) (18),Sheep (Ovine) (17),Zebrafish (7),Guinea Pig (5),Primate (4),Monkey (3),Rabbit (3),Goat (2),Non-Human Primate (1),Zebrafish (Brachydanio rerio) (1)
宿主
其他选择 Mouse
Mouse(700),Rat (492),Rabbit (138),Goat (2),Chicken (1)
克隆类型 (克隆位点)
单克隆 (LT40)
标记
其他选择 FITC
FITC(194),PE (150),Biotin (134),APC (64),Cy5 (21),PerCP (16),PE-Cy7 (13),SPRD (11),Cy5,PE (10),PE,Cy5 (10),PE-Cy5.5 (10),PE-Cy5 (9),Cy7,PE (8),Allophycocyanin (7),PerCP,Cy5.5 (7),APC-Cy7 (6),Alexa Fluor 488 (6),Alexa Fluor 647 (6),HRP (6),DyLight 488 (5),DyLight 550 (5),DyLight 650 (5),APC-Cy5.5 (4),Alexa Fluor 350 (4),Alexa Fluor 555 (4),Alexa Fluor 700 (4),Cy3 (4),Cy5.5 (4),Cy7 (4),EFluor 450 (4),PE,Cy7 (4),APC eFluor 780 (3),DyLight 594 (3),DyLight 680 (3),DyLight 755 (3),PE-TR (3),Alkaline Phosphatase (AP) (2),FLMA (2),Orange Cytognos 515 (2),PE-DyLight 594 (2),PE-Dyomics 590 (2),Texas Red (TR) (2),Cy5.5,PE (1),DY647 (1),Dyomics 647 (1),His tag (1),PE,DY590 (1),PE,DY647 (1),PE,Dyomics 647 (1),PE-Dyomics 647 (1),SureLight® R-PE (1),TRI-COLOR (TC) (1)
应用范围
其他选择 Flow Cytometry (FACS)
Flow Cytometry (FACS)(1070),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (212),Immunoprecipitation (IP) (212),Immunohistochemistry (Frozen Sections) (IHC (fro)) (209),Western Blotting (WB) (196),Immunohistochemistry (IHC) (159),Immunofluorescence (IF) (107),ELISA (74),Immunocytochemistry (ICC) (63),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (55),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (40),Functional Studies (Func) (22),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (8),Enzyme Immunoassay (EIA) (6),Cytotoxicity Test (CyTox) (3),Direct Fluorescent Assay (DFA) (2),Immunohistochemistry (Acetone-fixed) (IHC (af)) (2),Indirect Immunofluorescence Assay (IFA) (2),Radioimmunoassay (RIA) (2),Cellular Assay (CA) (1),Microarray (MA) (1)
[Pubmed] 2 references available
规格 0.5 mg
发货至 ZG (更改)
发货时间 23至28个工作日

PTPRC抗体

产品细节

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产品细节

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克隆位点 LT40
亚型 IgM
产品特性 The antibody detects: Chicken CD45 (Mr 190 to 215 kDa).
The ability of MAb LT40 to discriminate this CD45lo subpopulation provides a suitable marker for the CD3-CD4-CD8lo immediate precursors to the CD3-CD4+CD8+ thymocytes.

目标详细情况

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目标详细情况

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别名 CD45
背景 Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45.1 Levels of CD45 expression detected by MAb LT40 in the avian thymus are heterogeneous with approximay 90 % of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10 % (CD45lo) of thymocytes. The CD45lo population contains exclusively thymocytes with the CD3-CD4-CD8lo phenotype characteristic of the immediate precursors to the CD3-CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8lo to CD4+CD8+ and before the expression of CD3.
研究领域 Immunology, Adaptive Immunity, Hematopoietic Progenitors, Glia marker, CD Antigens, Surface Receptors of Immune Cells

使用细节

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应用备注 Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
样本量 1 mL
限制 For Research Use only

贮存及处理

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贮存及处理

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缓冲液 Product is supplied as 0.5 mg in 1.0 mL of PBS / NaN3.
储存液 Sodium azide
注意事项 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediay. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事项 Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
储存条件 4 °C

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