Alstrom Syndrome 1 （ALMS1） （C-Term）...

Alstrom Syndrome 1 (ALMS1) (C-Term) 抗体

抗原
Alstrom Syndrome 1 (ALMS1)
别名 ALMS1, ALSS, bbb
抗原表位
其他选择 C-Term
C-Term(7),Internal Region (6),AA 1200-1250 (2),AA 3400-3450 (1),AA 4104-4167 (1)
适用
其他选择 Human
Human(19),Dog (Canine) (2),Murine (2),Bovine (1),Rat ((Rattus)) (1)
宿主
其他选择 Goat
Rabbit(16),Goat (5)
克隆类型
多克隆
实验类型 Peptide ELISA
应用范围
其他选择 ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Western Blotting (WB)(15),ELISA (13),Immunohistochemistry (IHC) (8),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4),Immunocytochemistry (ICC) (2),Immunofluorescence (IF) (2),Immunoprecipitation (IP) (2),Enzyme Immunoassay (EIA) (1),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (1),Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
[Pubmed] 1 reference available
规格 100 μg
发货至 ZG (更改)
发货时间 24至29个工作日

Alstrom Syndrome 1 (ALMS1) (C-Term) 抗体

产品细节

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免疫原 Peptide with sequence C-RVTNQLLGRKVPWD, from the C Terminus of the protein sequence according to NP_055935.4.
特异性 The C terminus of ALMS1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis.
交叉反应 Murine, Rat ((Rattus)), Dog (Canine), Bovine
纯化方法 Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Alstrom Syndrome 1 (ALMS1) (C-Term) 抗体目标详细情况

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目标详细情况

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别名 ALMS1
背景 Synonyms: ALMS1, Alstrom syndrome 1, ALSS, KIAA0328, DKFZp686A118, DKFZp686D1828
基因ID 7840, 236266, 297408
NCBI Accession NP_055935
研究领域 Immunology

使用细节

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应用备注 ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 200 kDa and 75 kDa in lysates of Human T cell line Jurkat after 0.3 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461 kDa according to NP_055935.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Testis shows staining of cytoplasm in spermatocytes. Recommended concentration: 1-3 µg/mL.
限制 For Research Use only

贮存及处理

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贮存及处理

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状态 Liquid
缓冲液 Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液 Sodium azide
Alstrom Syndrome 1 (ALMS1) (C-Term) 抗体注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Minimize freezing and thawing.
储存条件 -20 °C
储存方法 Aliquot and store at -20 °C.

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