A9 A9 小鼠皮下结缔组织细胞

A9 小鼠皮下结缔组织细胞ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞。细胞库管理规范，提供的细胞株背景清楚，提供参考文献和优培养条件

A9 小鼠皮下结缔组织细胞

ATCC® Number: CCL-1.4™

Designations: A9 (APRT and HPRT negative derivative of Strain L)

Depositors: TR Chen

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus (mouse)

Morphology: fibroblast

A9 小鼠皮下结缔组织细胞

Source: Strain: C3H/An

Tissue: subcutaneous connective tissue; areolar and adipose

Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Tumorigenic: Yes

Antigen expression: H-2k

Cytogenetic Analysis: modal number = 52; range = 46 to 54; This is a hyperdiploid cell line with 24 to 26 marker chromosomes common to all cells., Nine markers (M1 - M9) are common to L-M(TK-) (see ATCC CCL-1.3) and 16 to 17 are found only in A9. The latter markers include Rb(3.5), Rb(4.15), Rb(5.5), T(8;19), Rb(9.17) and 11 to 12 others., Structurally normal chromosomes 4, 15, 16, 17, 18 and X were absent.

Age: 100 days

Gender: male

Comments: The A9 cell line was isolated by J.W. Littlefield from wild-type Strain L cells.

The cells are sensitive to HAT selection medium and deficient in adenosine phosphoribosyl transferase (APRT) and hypoxanthine phosphoribosyl transferase (HPRT).

The cells are resistant to 8-azaguanine (0.003 mg/ml) and 6-thioguanine (0.1 mM).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5g/L glucose, 90%; fetal bovine serum, 10%

A9 小鼠皮下结缔组织细胞

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days

Remove old medium, add fresh 0.25% trypsin, EDTA (0.03%) solution, rinse and remove. Allow cells to sit at room temperature (or at 37C) until cells detach (about 5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Related Products: parental cell line:ATCC CCL-1

References: 21409: Littlefield JW. The inosinic acid pyrophosphorylase activity of mouse fibroblasts partially resistant to 8-azaguanine. Proc. Natl. Acad. Sci. USA 50: 568-573, 1963. PubMed: 14067106

21410: Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673

27669: Mayr GA, Freimuth P. A single locus on human chromosome 21 directs the expression of a receptor for adenovirus type 2 in mouse A9 cells. J. Virol. 71: 412-418, 1997. PubMed: 8985365

A9 小鼠皮下结缔组织细胞

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iec-6 大鼠小肠隐窝上皮细胞

马-达二氏犬肾细胞系 MDCK (NBL-2) CRL-34

鸭子胚胎成纤维细胞　 Duck embryo　 CCL-141

小鼠胚胎成纤维细胞 3T3-L1 CCL-173

人结肠直肠腺癌 COLO 320DM CCL-220

人结肠直肠腺癌 NCI-H508 CRL-253

人结肠直肠腺癌 COLO 320HSR CCL-220.1

人B淋巴细胞 Ramos（RA1） CRL-1596

人淋巴母细胞 T2(174×CEM.T2) CRL-1992

大鼠心肌细胞 H9C2(2-1) CRL-1446

A9 小鼠皮下结缔组织细胞

猪鼻甲黏膜成纤维细胞 PT-K75 CRL-2528

小鼠癌细胞 LTPA CRL-2389

大鼠癌细胞 DSL-6A/C1 CRL-2132

小鼠瘤B淋巴细胞 SP2/MIL-6 CRL-2016

大鼠脑间质细胞 DI TNC1 CRL-2005

小鼠肌原细胞 C2C12 CRL-1772

鹌鹑纤维肉瘤细胞 QT6 CRL-1708

大鼠回肠细胞 IEC-18 CRL-1589

非洲绿猴肾细胞 VERO C1008 [Vero 76, clone E6, Vero E6] CRL-1586

人成纤维细胞 Hs 738.St/Int CRL-7869

人正常前列腺上皮细胞 WPE-int CRL-2888

人正常前列腺成肌纤维细胞 WPMY-1 CRL-2854

人导管腺癌细胞 PL45 CRL-2558

人癌细胞 Panc 05.04 CRL-2557

人癌细胞 Panc 04.03 CRL-2555

人癌细胞 Panc 02.03 CRL-2553

人癌细胞 Panc 08.13 CRL-2551

人癌细胞 Panc 03.27 CRL-2549

人癌细胞 Panc 10.05 CRL-2547

人肝癌细胞 SNU-398 CRL-2233

人成神经瘤细胞- SK-N-DZ CRL-2149

人癌细胞 HPAC CRL-2119

人癌细胞 HPAF-II CRL-1997

人黑色素瘤细胞 A375.S2 CRL-1872

人脐静脉内皮细胞 HUV-EC-C CRL-1730

人黑素瘤细胞 WM-266-4 CRL-1676

人膀胱癌细胞 HT-1376 CRL-1472

人正常前列腺上皮细胞 RWPE-2 CRL-11610

人正常前列腺上皮细胞 RWPE-1 CRL-11609

鸡胚胎成纤维细胞 UMNSAH/DF-1 CRL-12203

人乳腺癌细胞 MDA-MB-231 HTB-26

A9 小鼠皮下结缔组织细胞

小鼠巨噬细胞 RAW 264.7 TIB-71

人横纹肌肉瘤细胞 A-204 HTB-82

人癌细胞 Capan-2 HTB-80

人癌细胞 Capan-1 HTB-79

人乳腺导管癌细胞 MDA-MB-435S HTB-129

人胃癌细胞 Hs 746T HTB-135

人肺腺癌细胞 NCI-H441 [H441] HTB-174

人瘤细胞 U266 TIB-196

人结肠癌细胞 NCI-H716 [H716] CCL-251

人多形性恶性胶质瘤 T98G [T98-G] CRL-1690

人神经母细胞瘤细胞 BE(2)-C CRL-2268

人肾透明细胞癌细胞 Caki-2 HTB-47

人皮肤成纤维细胞 Hs 865.Sk CRL-7601

CA46 人淋巴瘤细胞系

CRL-5826 NCI-H226 人肺鳞癌细胞系

Tca-8113 人舌鳞癌细胞

MA-891 小鼠乳腺癌高转移细胞

U14 小鼠宫颈癌细胞

CRL-9518 L5178Y 小鼠淋巴瘤细胞

HTB-112 HEC-1-A 人子宫内膜腺癌细胞

HTB-113 HEC-1-B 人子宫内膜腺癌细胞

AN3 CA 人子宫内膜腺癌细胞

ROS1728 大鼠成骨细胞

CRL-6323 B16F1 小鼠黑色素瘤细胞

HTB-58 SK-MES-1 人肺鳞癌细胞

CCL-127 IMR-32 人神经母细胞瘤细胞

HTB-79 Capan-2 人癌细胞

BALL-1 人B淋巴细胞急性白血病细胞

A9 小鼠皮下结缔组织细胞

CRL-2922 Eayh926 人脐静脉细胞融合细胞

WB-F344 大鼠肝干细胞

CRL-1715 TM4 正常小鼠睾丸细胞

CRL-2539 4T1 小鼠乳腺癌细胞

CRL-1442 BRL-3A 大鼠肝细胞

BRL 大鼠肝细胞

RSC96大鼠雪旺细胞

Isk 人子宫内膜癌细胞

HTB-113 hec-1-b 人子宫内膜腺癌细胞

PLA-802?人恶性横纹肌肉瘤

Pt-K2 袋鼠肾细胞

BS-C-1 非洲绿猴肾细胞

A498人肾癌细胞

3AO人卵巢癌细胞

iec-6 大鼠小肠隐窝上皮细胞

u87MG 人脑星形胶质母细胞瘤

Renca 小鼠肾癌细胞

nci-h929 瘤细胞

P388小鼠白血病细胞

A9 小鼠皮下结缔组织细胞

opm-2 瘤细胞

MDA-MB-435 人乳腺癌高转移细胞

KU812 人外周血嗜碱性白细胞

NIH:OVCAR-3 人卵巢癌细胞

SW 1990人癌细胞系

S180人结肠腺癌细胞

HuTu-80十二指肠腺癌

RL95-2 人子宫内腺癌细胞

NCI-H446 人小细胞肺癌细胞

MSTO-211H 人肺癌细胞

H4-Ⅱe 大鼠肝癌细胞系

HLF 人肺成纤维样细胞

LS 180人结肠腺癌细胞

HuTu-80十一指肠腺癌

ST猪睾丸细胞系

REH人急性非B非T淋巴细胞白血病

NCI-H520 人肺鳞癌细胞

RKO 人结肠腺癌细胞

293A 人胚肾细胞

RK-13 兔肾细胞系