Catalogue Number | CMV-217 |
Introduction | CMV belongs to the Betaherpesvirinae subfamily of Herpesviridae which includes herpes simplex virustypes 1 and 2, varicella-zoster virus, and Epstein-Barrvirus. The herpesviruses share a characteristic ability to remain latentover long periods. CMV is a double-stranded linear DNA virus with 162 hexagonal protein capsomeres surrounded by a lipid membrane. CMV has the largest genome of the herpes viruses, ranging from 230-240 kilobase pairs. Human CMV is composed of unique and inverted repeats that include the existence of 4 genome isomers caused by inversion of L-S genome components (class E). Replication may be divided into immediate early, delayed early, and late gene expression based on time of synthesis after infection. The DNA is replicated by rolling circles. In vitro, CMV replicates in human fibroblasts. |
Description | The E.Coli derived recombinant artificial mosaic protein contains the CMV gB immunodominant regions, 60-81aa of gB protein strain AD169 repeated three times. |
Purification Method | CMV Mosaic protein was purified by proprietary chromatographic technique. |
Purity | CMV Mosaic protein is >95% pure as determined by 10% PAGE (coomassie staining). |
Formulation | 50mM Tris-Hcl pH 8.0, 60mM NaCl, 10mM glutathione 0.1% sarcosil and 50% glycerol. |
Storage | CMV Mosaic protein although stable at 4°C for 1 week, should be stored below -18°C.
Please prevent freeze thaw cycles. |
Specifivity | :Immunoreactive with sera of CMV-infected individuals. |
Applications | CMV Mosaic antigen is suitable for ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems. |
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |