Introduction | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. |
Description | Insulin Human Recombinant produced in Yeast is a two chain, glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5807 Dalton. Zinc content was found to be 0.4%. Insulin is purified by proprietary chromatographic techniques. |
Source | Saccharomyces cerevisiae. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation | The protein was lyophilized from 50mM Sodium Chloride solution. |
Solubility | It is recommended to reconstitute the lyophilized Insulin in PBS pH-7.5. |
Stability | Lyophilized Insulin should be stored at 4°C. Upon reconstitution Insulin should be stored at -20°C to -80°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
Purity | Greater than 98.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE. |
Biological Activity | 27 units/mg.
Insulin has been evaluated in cell culture (Human Foreskin Fibroblasts).
The effective concentration range for use in defined media is 1-5ug/ml. However, the optimum concentration range for a particular application must be determined by the investigator. |
Usage | ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |