小鼠孤腓肽酶免试剂盒,(OFQ/N)ELISA检测试剂盒 ,英文名:OFQ/N ELISA Kit ,英文缩写:OFQ/N ,常见试剂盒规格:96T/48T,产品别名:小鼠孤腓肽ELISA检测试剂盒,OFQ/N 检测试剂盒, OFQ/N 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠孤腓肽酶免试剂盒,(OFQ/N)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不一致,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
神经生长因子(NGF)检测试剂盒(化学发光免疫分析法)CLIA Kit for Nerve Growth Factor (NGF)96TY16946Rattus norvegicus (Rat)
神经营养因子3(NT3)检测试剂盒(化学发光免疫分析法)CLIA Kit for Neurotrophin 3 (NT3)96TY16947Homo sapiens (Human)
骨保护素(OPG)检测试剂盒(化学发光免疫分析法)CLIA Kit for Osteoprotegerin (OPG)96TY16948Homo sapiens (Human)
骨保护素(OPG)检测试剂盒(化学发光免疫分析法)CLIA Kit for Osteoprotegerin (OPG)96TY16949Mus musculus (Mouse)
抑瘤素M(OSM)检测试剂盒(化学发光免疫分析法)CLIA Kit for Oncostatin M (OSM)96TY16950Homo sapiens (Human)
白介素12(IL12)检测试剂盒(化学发光免疫分析法)CLIA Kit for Interleukin 12 (IL12)96TY16951Homo sapiens (Human)
调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒(化学发光免疫分析法)CLIA Kit for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)96TY16952Homo sapiens (Human)
调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒(化学发光免疫分析法)CLIA Kit for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)96TY16953Mus musculus (Mouse)
干细胞因子(SCF)检测试剂盒(化学发光免疫分析法)CLIA Kit for Stem Cell Factor (SCF)96TY16954Homo sapiens (Human)
干细胞因子(SCF)检测试剂盒(化学发光免疫分析法)CLIA Kit for Stem Cell Factor (SCF)96TY16955Mus musculus (Mouse)
干细胞因子受体(SCFR)检测试剂盒(化学发光免疫分析法)CLIA Kit for Stem Cell Factor Receptor (SCFR)96TY16956Homo sapiens (Human)
转化生长因子α(TGFα)检测试剂盒(化学发光免疫分析法)CLIA Kit for Transforming Growth Factor Alpha (TGFa)96TY16957Homo sapiens (Human)
转化生长因子β1(TGFβ1)检测试剂盒(化学发光免疫分析法)CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1)96TY16958Homo sapiens (Human)
转化生长因子β1(TGFβ1)检测试剂盒(化学发光免疫分析法)CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1)96TY16959Mus musculus (Mouse)
转化生长因子β1(TGFβ1)检测试剂盒(化学发光免疫分析法)CLIA Kit for Transforming Growth Factor Beta 1 (TGFb1)96TY16960Rattus norvegicus (Rat)
组织金属蛋白酶YZ因子3(TIMP3)检测试剂盒(化学发光免疫分析法)CLIA Kit for Tissue Inhibitors Of Metalloproteinase 3 (TIMP3)96TY16961Homo sapiens (Human)
肿瘤坏死因子α(TNFα)检测试剂盒(化学发光免疫分析法)CLIA Kit for Tumor Necrosis Factor Alpha (TNFa)96TY16962Bos taurus; Bovine (Cattle)
肿瘤坏死因子α(TNFα)检测试剂盒(化学发光免疫分析法)CLIA Kit for Tumor Necrosis Factor Alpha (TNFa)96TY16963Homo sapiens (Human)
肿瘤坏死因子α(TNFα)检测试剂盒(化学发光免疫分析法)CLIA Kit for Tumor Necrosis Factor Alpha (TNFa)96TY16964Mus musculus (Mouse)
肿瘤坏死因子α(TNFα)检测试剂盒(化学发光免疫分析法)CLIA Kit for Tumor Necrosis Factor Alpha (TNFa)96TY16965Rattus norvegicus (Rat)
血小板生成素(TPO)检测试剂盒(化学发光免疫分析法)CLIA Kit for Thrombopoietin (TPO)96TY16966Homo sapiens (Human)
肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒(化学发光免疫分析法)CLIA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)96TY16967Homo sapiens (Human)
尿激酶型纤溶酶原激活因子(uPA)检测试剂盒(化学发光免疫分析法)CLIA Kit for Plasminogen Activator, Urokinase (uPA)96TY16968Homo sapiens (Human)
血管内皮生长因子A(VEGFA)检测试剂盒(化学发光免疫分析法)CLIA Kit for Vascular Endothelial Growth Factor A (VEGFA)96TY16969Canis familiaris; Canine (Dog)
血管内皮生长因子A(VEGFA)检测试剂盒(化学发光免疫分析法)CLIA Kit for Vascular Endothelial Growth Factor A (VEGFA)96TY16970Homo sapiens (Human)
血管内皮生长因子A(VEGFA)检测试剂盒(化学发光免疫分析法)CLIA Kit for Vascular Endothelial Growth Factor A (VEGFA)96TY16971Mus musculus (Mouse)
血管内皮生长因子A(VEGFA)检测试剂盒(化学发光免疫分析法)CLIA Kit for Vascular Endothelial Growth Factor A (VEGFA)96TY16972Rattus norvegicus (Rat)
血管内皮生长因子C(VEGFC)检测试剂盒(化学发光免疫分析法)CLIA Kit for Vascular Endothelial Growth Factor C (VEGFC)96TY16973Homo sapiens (Human)
激肽释放酶3(KLK3)检测试剂盒(化学发光免疫分析法)CLIA Kit for Kallikrein 3 (KLK3)96TY16974Homo sapiens (Human)
甲胎蛋白(αFP)检测试剂盒(化学发光免疫分析法)CLIA Kit for Alpha-Fetoprotein (aFP)96TY16975Homo sapiens (Human)
糖抗原15-3(CA15-3)检测试剂盒(化学发光免疫分析法)CLIA Kit for Carbohydrate Antigen 15-3 (CA15-3)96TY16976Homo sapiens (Human)
糖抗原19-9(CA19-9)检测试剂盒(化学发光免疫分析法)CLIA Kit for Carbohydrate Antigen 19-9 (CA19-9)96TY16977Homo sapiens (Human)
血小板因子4(PF4)检测试剂盒(化学发光免疫分析法)CLIA Kit for Plaet Factor 4 (PF4)96TY16978Homo sapiens (Human)
血小板因子4(PF4)检测试剂盒(化学发光免疫分析法)CLIA Kit for Plaet Factor 4 (PF4)96TY16979Mus musculus (Mouse)
糖化血红蛋白(HbA1C)检测试剂盒(化学发光免疫分析法)CLIA Kit for Glycated Hemoglobin (HbA1C)96TY16980Homo sapiens (Human)
纤维蛋白原(FG)检测试剂盒(化学发光免疫分析法)CLIA Kit for Fibrinogen (FG)96TY16981Homo sapiens (Human)
髓细胞触发受体1(TREM1)检测试剂盒(化学发光免疫分析法)CLIA Kit for Triggering Receptor Expressed On Myeloid Cells 1 (TREM1)96TY16982Homo sapiens (Human)
α1-微球蛋白(α1M)检测试剂盒(化学发光免疫分析法)CLIA Kit for Alpha-1-Microglobulin/Bikunin Precursor (a1M)96TY16983Homo sapiens (Human)
牛血清白蛋白(BSA)检测试剂盒(化学发光免疫分析法)CLIA Kit for Bovine Serum Albumin (BSA)96TY16984General
胰蛋白酶(TRY)检测试剂盒(化学发光免疫分析法)CLIA Kit for Trypsin (TRY)96TY16985Sus scrofa; Porcine (Pig)
β2-微球蛋白(β2M)检测试剂盒(化学发光免疫分析法)CLIA Kit for Beta-2-Microglobulin (b2M)96TY16986Homo sapiens (Human)
胰岛素样生长因子结合蛋白6(IGFBP6)检测试剂盒(化学发光免疫分析法)CLIA Kit for Insulin Like Growth Factor Binding Protein 6 (IGFBP6)96TY16987Mus musculus (Mouse)
甲状腺结合球蛋白(TBG)检测试剂盒(化学发光免疫分析法)CLIA Kit for Thyroxine Binding Globulin (TBG)96TY16988Homo sapiens (Human)
抗白蛋白抗体(AAA)检测试剂盒(化学发光免疫分析法)CLIA Kit for Anti-Albumin Antibody (AAA)96TY16989Homo sapiens (Human)
甲状腺球蛋白(TG)检测试剂盒(化学发光免疫分析法)CLIA Kit for Thyroglobulin (TG)96TY16990Homo sapiens (Human)
凝溶胶蛋白(GS)检测试剂盒(化学发光免疫分析法)CLIA Kit for Gelsolin (GS)96TY16991Homo sapiens (Human)
补体3a(C3a)检测试剂盒(化学发光免疫分析法)CLIA Kit for Complement Component 3a (C3a)96TY16992Homo sapiens (Human)
补体成分5a(C5a)检测试剂盒(化学发光免疫分析法)CLIA Kit for Complement Component 5a (C5a)96TY16993Mus musculus (Mouse)
P物质(SP)检测试剂盒(化学发光免疫分析法)CLIA Kit for Substance P (SP)96TY16994Bos taurus; Bovine (Cattle)
P物质(SP)检测试剂盒(化学发光免疫分析法)CLIA Kit for Substance P (SP)96TY16995Canis familiaris; Canine (Dog)
各种类型ELISA测定时,一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。