小鼠粘蛋白/粘液素7酶免试剂盒,(MUC7)ELISA检测试剂盒 ,英文名:MUC7 ELISA Kit ,英文缩写:MUC7 ,常见试剂盒规格:96T/48T,产品别名:小鼠粘蛋白/粘液素7ELISA检测试剂盒,MUC7 检测试剂盒, MUC7 检测试剂盒
ELISA实验操作较繁杂,操作不当将引起较大的误差。小鼠粘蛋白/粘液素7酶免试剂盒,(MUC7)ELISA检测试剂盒在操作中应注意以下5个方面。
1.随着病情的进展或由其他因素影响,某些抗体在机体内的含量发生大幅波动,因此有必要对标本进行预处理,例如对血清标本作适当稀释,或离心分离血脂等。间接法测定中,标本适当稀释还可降低非特异性反应。
2.加样一般使用加液器,在ELISA中一般有4次加样:加标本、加酶结合物、加底物和加终止液。加标本时必须每份标本使用一支移液器吸嘴,以免交叉污染。加酶结合物、底物和终止液时则不需更换吸嘴。
3.在成批手工检测中,还应注意操作时差的影响。加样及混匀时间的差异,使抗原抗体反应和酶促反应时间不一致,对竞争法及定量检测影响很大,不注意可能会导致错误结果或定量不准。
4.洗涤是ELISA操作过程中决定实验成败的一个关键步骤。目的是除去未结合的免疫反应物,终止抗原抗体反应,除去标本中与反应无关的成分、游离的酶标物以及反应过程中吸附于固相载体上的非特异性物质。可用洗板机洗板或手工洗板,前者洗涤质量较好,各反应孔洗涤效果均一,批内、批间差异小,手工洗板应注意控制各孔洗涤效果,差异不宜太大。
5.准确判定结果须使用ELISA测读仪(酶标仪),比色法判读可选择单波长或双波长,根据反应液颜色的不同选用不同波长的滤光片,以空白孔校零测定反应孔的吸光度值(A值)。酶标仪具有良好的重复性。
乳腺丝抑蛋白(Maspin)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Maspin48T/96TY13135Homo sapiens (Human)
几丁质酶1(CHIT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chitinase 1 (CHIT1)48T/96TY13136Mus musculus (Mouse)
Zeste同源物增强子2(EZH2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Enhancer Of Zeste Homolog 2 (EZH2)48T/96TY13137Homo sapiens (Human)
微小染色体维持缺陷蛋白5(MCM5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Minichromosome Maintenance Deficient 5 (MCM5)48T/96TY13138Homo sapiens (Human)
肝配蛋白A4(EFNA4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Ephrin A4 (EFNA4)48T/96TY13139Homo sapiens (Human)
EB病毒诱导蛋白3(EBI3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Epstein Barr Virus Induced Protein 3 (EBI3)48T/96TY13140Mus musculus (Mouse)
前妊娠蛋白关联子宫内膜蛋白(PAEP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Progestagen Associated Endometrial Protein (PAEP)48T/96TY13141Homo sapiens (Human)
淋巴细胞抗原75(LY75)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Lymphocyte Antigen 75 (LY75)48T/96TY13142Mus musculus (Mouse)
CD276分子(CD276)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 276 (CD276)48T/96TY13143Homo sapiens (Human)
信号素3E(SEMA3E)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Semaphorin 3E (SEMA3E)48T/96TY13144Mus musculus (Mouse)
小白蛋白(PVALB)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Parvalbumin (PVALB)48T/96TY13145Rattus norvegicus (Rat)
衰老关键蛋白4(FBLN4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fibulin 4 (FBLN4)48T/96TY13146Homo sapiens (Human)
Sideroflexin 1蛋白(SFXN1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Sideroflexin 1 (SFXN1)48T/96TY13147Mus musculus (Mouse)
SRC/FGR/YES相关FYN癌基因(FYN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for FYN Oncogene Related To SRC/FGR/YES (FYN)48T/96TY13148Mus musculus (Mouse)
染色质装配因子1亚基B(CHAF1B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Chromatin Assembly Factor 1, Subunit B (CHAF1B)48T/96TY13149Mus musculus (Mouse)
氨酰tRNA合成酶复合多功能相互作用蛋白1(AIMP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1)48T/96TY13150Homo sapiens (Human)
氨酰tRNA合成酶复合多功能相互作用蛋白1(AIMP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1)48T/96TY13151Mus musculus (Mouse)
碱性唾液脯氨酸丰富蛋白2(PRB2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Basic Salivary Proline Rich Protein 2 (PRB2)48T/96TY13152Homo sapiens (Human)
碱性唾液脯氨酸丰富蛋白1(PRB1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Basic Salivary Proline Rich Protein 1 (PRB1)48T/96TY13153Homo sapiens (Human)
Dickkopf相关蛋白4(DKK4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dickkopf Related Protein 4 (DKK4)48T/96TY13154Homo sapiens (Human)
Dickkopf相关蛋白4(DKK4)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Dickkopf Related Protein 4 (DKK4)48T/96TY13155Mus musculus (Mouse)
天冬氨酸β羟化酶(ASPH)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Aspartate Beta Hydroxylase (ASPH)48T/96TY13156Homo sapiens (Human)
分泌型卷曲相关蛋白2(SFRP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Secreted Frizzled Related Protein 2 (SFRP2)48T/96TY13157Homo sapiens (Human)
序列相似家族3成员B(FAM3B)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Family With Sequence Similarity 3, Member B (FAM3B)48T/96TY13158Mus musculus (Mouse)
含胶原三螺旋重复蛋白1(CTHRC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1)48T/96TY13159Homo sapiens (Human)
AE结合蛋白1(AEBP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for AE Binding Protein 1 (AEBP1)48T/96TY13160Homo sapiens (Human)
微管蛋白β1(TUBβ1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Tubulin Beta 1 (TUBb1)48T/96TY13161Mus musculus (Mouse)
酶原颗粒膜糖蛋白(GP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Glycoprotein 2, Zymogen Granule Membrane (GP2)48T/96TY13162Rattus norvegicus (Rat)
花生四烯酸脂加氧酶3(ALOXE3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Arachidonate Lipoxygenase 3 (ALOXE3)48T/96TY13163Homo sapiens (Human)
癌胚抗原相关细胞粘附分子6(CEACAM6)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Carcinoembryonic Antigen Related Cell Adhesion Molecule 6 (CEACAM6)48T/96TY13164Homo sapiens (Human)
线粒体解偶联蛋白1(UCP1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Uncoupling Protein 1, Mitochondrial (UCP1)48T/96TY13165Rattus norvegicus (Rat)
F-框亮氨酸丰富重复蛋白3(FBXL3)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for F-Box And Leucine Rich Repeat Protein 3 (FBXL3)48T/96TY13166Mus musculus (Mouse)
脂肪酸合酶(FASN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Fatty Acid Synthase (FASN)48T/96TY13167Mus musculus (Mouse)
金属硫蛋白1M(MT1M)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Metallothionein 1M (MT1M)48T/96TY13168Homo sapiens (Human)
Vasorin蛋白(VASN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Vasorin (VASN)48T/96TY13169Homo sapiens (Human)
精胺氧化酶(SMOX)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Spermine Oxidase (SMOX)48T/96TY13170Mus musculus (Mouse)
丝氨酸棕榈酰转移酶长链碱性亚基1(SPTLC1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1)48T/96TY13171Mus musculus (Mouse)
基质金属蛋白酶9(MMP9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 9 (MMP9)48T/96TY13172Canis familiaris; Canine (Dog)
5羟色胺转运蛋白(SERT)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Serotonin Transporter (SERT)48T/96TY13173Rattus norvegicus (Rat)
3-羟基3-甲基戊二酰辅酶A还原酶(HMGCR)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR)48T/96TY13174Rattus norvegicus (Rat)
钙调蛋白样蛋白5(CALML5)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Calmodulin Like Protein 5 (CALML5)48T/96TY13175Homo sapiens (Human)
软骨关联蛋白(CRTAP)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cartilage Associated Protein (CRTAP)48T/96TY13176Mus musculus (Mouse)
AF4/FMR2家族成员1(AFF1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for AF4/FMR2 Family, Member 1 (AFF1)48T/96TY13177Homo sapiens (Human)
白介素1家族成员9(IL1F9)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interleukin 1 Family, Member 9 (IL1F9)48T/96TY13178Mus musculus (Mouse)
基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Matrix Metalloproteinase 2 (MMP2)48T/96TY13179Sus scrofa; Porcine (Pig)
次黄嘌呤磷酸核糖基转移酶1(HPRT1)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)48T/96TY13180Homo sapiens (Human)
WNTYZ因子1(WIF1)检测试剂盒(酶联免疫吸附试验法) ELISA Kit for WNT Inhibitory Factor 1 (WIF1)48T/96TY13181Homo sapiens (Human)
肾足蛋白(NPHN)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Nephrin (NPHN)48T/96TY13182Rattus norvegicus (Rat)
CD200分子(CD200)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Cluster Of Differentiation 200 (CD200)48T/96TY13183Homo sapiens (Human)
干扰素α/β受体2(IFNα/βR2)检测试剂盒(酶联免疫吸附试验法)ELISA Kit for Interferon Alpha/Beta Receptor 2 (IFNa/bR2)48T/96TY13184Homo sapiens (Human)
各种类型ELISA测定时, 一般需经过以下步骤:固相包被→加待测样品→温育→洗涤+加酶标物→温育→洗涤→加底物→温育一加终止液→比色→判定结果。