Human GDNF/重组人神经胶质源神经营养因子

Human GDNF/重组人神经胶质源神经营养因子
产品编号：100-030
产品规格：10μg
Description: GDNF is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRalpha (alpha1-alpha4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson's disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer, of two 15 kDa polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which (Cys 101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration.
Source: E. coli
Molecular Weight: 30.0 kDa
Purity: > 98% by SDS-PAGE analyses
Endotoxin level/内毒素水平: < 0.1 ng per g of GDNF
Stabilizer: none
Formulation: lyophilized
Biological Activity:
Assay #1: Determined by the dose-dependent dopamine uptake by rat mesencephalic cultures was found to be in the range of 5-10 ng/ml.
Assay #2:The was determined by the proliferation of rat C6 cells is < 0.1 ng/ml, corresponding to a specific activity of > 1 x 107 units/mg.
Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -200C. Reconstituted GDNF is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C. Avoid repeated freeze/thaw cycles.
Avoid repeated freeze-thaw cycles.
Usage: Human GDNF is offered for research use. Not for drug use. Not for human use.