Human sCD40 Ligand/TRAP/重组人可溶性sCD40配体/TRAP

Human sCD40 Ligand/TRAP/重组人可溶性sCD40配体/TRAP
产品编号：100-115
产品规格：50μg 
Description: CD40, a member of the TNF receptor family, is a cell surface protein expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The human CD40L gene codes for a 261 amino acid type II transmembrane protein, which contains a 22 amino acid cytoplasmic domain, a 24 amino acid transmembrane domain, and a 215 amino acid extracellular domain. The soluble form of CD40L is an 18 kDa protein comprising the entire TNF homologous region of CD40L and is generated in vivo by an intracellular proteolytic processing of the full length CD40L. Recombinant human soluble CD40 ligand is a 16.3 kDa protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40L.
Source: E. coli
Molecular Weight: 16.3 kDa
Purity: > 97 % (SDS-PAGE; HPLC)
Endotoxin level./内毒素水平: < 0.1 ng per μg of sCD40L/TRAP
Stabilizer: none
Formulation: lyophilized
Biological Activity:
Assay #1: Determined by the stimulation of IL-12 induction by human PBMCs. Assay #2: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The expected for this effect is 5-10 ng/ml. Note: Results may vary with PBMC donors.
Stability: The lyophilized protein is stable for at least 2 years from date of receipt at -200C. Reconstituted sCD40L is stable for at least 3 months when stored in working aliquots with a carrier protein at -200C. Avoid repeated freeze/thaw cycles.
Avoid repeated freeze-thaw cycles.
Usage: Human sCD40L/TRAP is offered for research use. Not for drug use. Not for human use.