Human Podoplanin (Fragment), soluble/重组人可溶性平足蛋白(Fragment)

Human Podoplanin (Fragment), soluble/重组人可溶性平足蛋白(Fragment)
产品编号：300-053
产品规格：5μg 
Description: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1-alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC-2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC-2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth. The recombinant soluble Podoplanin starts with GLST and ends with GLST.
Source: E. coli
Molecular Weight: 25 kDa
Purity: > 95%, by SDS-PAGE and visualised by silver stain
Endotoxin level/内毒素水平: < 0.1 ng per μg of Podoplanin
Stabilizer: none
Buffer: PBS
Formulation: lyophilized
Reconstitution: We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.
Stability: The lyophilized protein is stable for a few weeks at room temperature, but best stored at –20oC. Reconstituted Podoplanin should be stored in working aliquots at –20oC. Avoid repeated freeze-thaw cycles.
Usage: Human Podoplanin is offered for research use. Not for drug use. Not for human use.