Human FGF-basic (FGF-2)/重组人碱性成纤维细胞生长因子-2
产品编号:300-001/300-002/300-003
产品规格:10μg/25μg/50μg
Description: The FGF family is composed of at least 23 polypeptides that show a variety of biological activities towards cells of mesenchymal, neuronal and epithelial origin. All members are heparin-binding growth factors (HB-GF). Until the structure of basic fibroblast growth factor (bFGF/FGF-2) was determined, a number of synonyms was used to describe this growth factor. As is often the case, the nomenclature reflected the observed activities reported by individual groups. Basic FGF has been reported as leukemia growth factor, macrophage growth factor, endothelial growth factor and tumor angiogenesis factor.
The eventual isolation and characterization of bFGF was done from soluble brain extracts. bFGF was found to have a molecular mass of 16.5 kDa and to be 154 amino acids in length. Interestingly, bFGF contains no hydrophobic leader sequence previously thougth to be required for cell secretion. Basic FGF bears 55% homology to acidic FGF and also seems to exist in three forms: the 154 amino-acid form and two other truncated versions of 146 and 131 amino acids lacking the N-terminal 9 and 24 residues.
Acidic and basic FGF compete for the binding to 125 kDa and 145 kDa receptor species. However, acidic FGF has a higher affinity for the 125 kDa species, while basic FGF has a higher affinity for the 145 kDa species. FGF receptor activation leads to the activation of MAP kinase and protein kinase C. FGF’s induce the proliferative response in cells derived from mesoderm and neuroectoderm. It seems that basic FGF reduces the average doubling time by shortening the G1 phase of the cell cycle. Furthermore, it has been reported to induce the release of plasminogen activator by endothelial cells. Perhaps one of the most potentially significant applications of bFGF is related to its reported ability to induce angiogenesis.
Source: E. coli
Molecular Weight: 16.5 kDa
Purity: > 95% by SDS-PAGE and visualised by silver stain
Endotoxin level/内毒素水平: < 0.1 ng per g of FGF-basic
Stabilizer: none
Buffer: PBS
Formulation: lyophilized
Biological Activity: The activity was determined by stimulation of 3H-thymidine incorporation in Balb/c 3T3 cells. The for this effect is ≤ 0.1 ng/ml corresponding to a specific activity of ≥ 1 x 107 units/mg. Maximal effect for DNA synthesis was observed at 1.0-2.0 ng/ml rh basic FGF.
Reconstitution: The lyophilized basic FGF should be reconstituted in water or 50 mM acetic acid containing at least 0.1% human or bovine serum albumin to a concentration not lower than 10 μg/ml.
Stability: Lyophilized samples are stable for greater than six months at -20°C to -70°C. Basic FGF can be stored in high-salt buffer (PBS, 1M NaCl) at 4°C for 2-4 weeks. Avoid repeated freeze-thaw cycles.
Usage: Human basic FGF is offered for research use. Not for drug use. Not for human use.