1each Adhesion Protein Protocols_详细资料

产品信息 A2719 Adhesion Protein Protocols - （Sigma） ISBN-10: 0-89603-417-8 ISBN-13: 978-0-89603-417-4 Description General description The cutting edge methods in this title will enable both novice and experienced researchers readily to identify and clone new adhesion proteins, to successfully produce inhibitors of the adhesive activity, to develop biological models for the assay of cell-to-matrix and cell-to-cell adhesion in vitro, and to measure the signaling activities of adhesion proteins. By illuminating these adhesive molecules and the possibilities for manipulating them, the new experimental strategies collected here will have considerable clinical potential for the regulation of immunity, inflammation, tissue remodeling, and embryonic development. 性质 publication info E. Dejana and M. Corada, ed., Humana Press, US, 1999, 240 pp., hard cover 目录表 Table of Contents Production of Monoclonals Directed to Adhesive Molecules. Monoclonal Antibodies Specific for Leucocyte Adhesion Molecules: Selective Protocols of Immunization and Screening Assays for Generation of Blocking, Activating, and Activation Reporter Antibodies, Epitope Mapping, Sequencing of Antibodies. Isolation and Cloning of Adhesive Molecules. Purification Through Affinity Chromatography and Microsequencing. Functional Analysis of Cell Adhesion Molecules, Application of Polymerase Chain Reaction for the Discovery of New Adhesion Molecule Family Members. Construction and Purification of Adhesion Molecule-Immunoglobulin Chimeric Proteins, Morphological Localization of Adhesive Molecules and Related Cytoskeletal Components. Immunofluorescence of Cultured Cells, Detection of Adhesion Molecules by Immunohistochemistry on Human and Murine Tissue Sections, In situ Hybridization with RNA Probes. Adhesion of Circulating Cells to the Endothelium and to Extracellular Matrix Proteins. Purification of Fibronectin and Vitronectin from Human Plasma, Adhesion to Matrix Proteins, Leucocyte-Endothelial Monolayer Adhesion Assay （Static Conditions）, Cell-Cell Adhesive Interactions in an In Vitro Flow Chamber, Adhesion of Tumor Cells to Endothelium Under Static Conditions. Adhesion of Tumor Cell Under Flow, Plaet Adhesion to the Subendothelium Under Flow. Plaet Adhesion to the Subendothelium Under Flow, Cell Chemotaxis. Leucocyte Transmigration Through Vascular Endothelium: An In Vitro Method, Cell Migration into a Wounded Area In Vitro. Endothelial Cell Permeability In Vitro. Determination of the Endothelial Barrier Function In Vitro. Induction of Intracellular Signals Through Binding of Adhesive Molecules. Activation of p125 FAK Tyrosine Phosphorylation, Induction of Ca²⁺ Flux by Adhesion Molecules in Lymphocytes. Integrin-Mediated Stimulation of Tyrosine Phosphorilation in Lymphoid Cells. The Identification of Signaling Molecules by the Yeast 2-Hybrid System, Index.

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